The effects of interferon-gamma and bacterial lipopolysaccharide on CD14 expression in human monocytes.
The effects of interferon-gamma and bacterial lipopolysaccharide on CD14 expression in human monocytes.
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干扰素-γ和细菌脂多糖对人单核细胞 CD14 表达的影响。
DOI：
10.1089/jir.1992.12.307
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Peluso,JF
中科院分区：
文献类型：
--
作者：
Payne,JB;Nichols,FC;Peluso,JF
CD14是脂多糖(LPS)-脂多糖结合蛋白受体。干扰素-γ(干扰素-γ)对CD14表达的影响尚未明确。本研究的目的是检测干扰素-γ单独和干扰素-γ联合细菌脂多糖对CD14表达的影响。采用逆流离心法分离人外周血单核细胞。单核细胞与干扰素-γ共同培养48h或与干扰素-γ共同培养24 h后再培养24 h，经流式细胞仪检测，单用干扰素-γ可下调未处理单核细胞CD14的表达。此外，在干扰素-γ预处理后，无论是中间普氏杆菌还是鼠伤寒沙门氏菌脂多糖，CD14的表达都显著下调。同样，单用干扰素-γ后，CD14+单核细胞的百分比下降，而在干扰素-γ治疗后，其下降幅度更大。这项研究清楚地表明，干扰素-γ下调CD14的表达，而干扰素-γ预处理后的脂多糖会增强这一作用。
CD14 has been reported to be the lipopolysaccharide (LPS)-LPS binding protein receptor. The effects of interferon-γ (IFN-γ) on CD14 expression have not been clearly established. The purpose of this investigation was to examine the effects of IFN-γ alone and IFN-γ followed by bacterial LPS on CD14 expression. Human peripheral blood monocytes were isolated by counterflow centrifugal elutriation (CCE). Monocytes were cultured for 48 h with IFN-γ alone or for 24 h with IFN-γ followed by LPS for a second 24 h. IFN-γ alone caused a down-regulation of CD14 expression, as assessed by flow cytometry, relative to CD14 expression in untreated monocytes. In addition, CD14 expression was even more significantly down-regulated after IFN-γ pretreatment followed by eitherPrevotella intermediaorSalmonella typhimuriumLPS. Likewise, the percentage of CD14+monocytes decreased after IFN-γ alone and even more dramatically after IFN-γ treatment followed by either LPS. This study clearly demonstrated that IFN-γ down-regulates CD14 expression and that LPS following IFN-γ pretreatment potentiates this effect.