Transdermal colorimetric patch for hyperglycemia sensing in diabetic mice
Transdermal colorimetric patch for hyperglycemia sensing in diabetic mice
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DOI：
10.1016/j.biomaterials.2020.119782
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发表时间：
2020-04-01
期刊：
影响因子：
14
通讯作者：
Gu, Zhen
中科院分区：
文献类型：
--
作者：
Wang, Zejun;Li, Hongjun;Gu, Zhen
采样和即时代谢物读数的集成可以从根本上提高患者的依从性。为了避免复杂的实验室仪器的需要，这里开发了一种一体化的经皮比色微针贴片，用于检测小鼠的高血糖。3，3‘，5，5’-四甲基联苯胺(TMB)的显色是由葡萄糖氧化酶(GOx)和辣根过氧化物酶(HRP)在异常高葡萄糖水平下的级联酶反应引发的。上层的HRP用磷酸钙(CAP)壳进行生物矿化，以增加pH响应功能，从而提高灵敏度并保护其免受非特异性反应的影响。这种比色传感器实现了对小鼠组织液的微创提取，并迅速将血糖水平转换为可见的颜色变化。通过扫描的微针图像可以获得定量的红、绿、蓝(RGB)信息。这种廉价的便携式比色传感器可以在不进行抽血程序的情况下检测日常血糖水平。
The integration of sampling and instant metabolite readout can fundamentally elevate patient compliance. To circumvent the need for complex in-lab apparatus, here, an all-in-one sampling and display transdermal colorimetric microneedle patch was developed for sensing hyperglycemia in mice. The coloration of 3,3',5,5'-tetramethylbenzidine (TMB) is triggered by the cascade enzymatic reactions of glucose oxidase (GOx) and horseradish peroxidase (HRP) at abnormally high glucose levels. The HRP in the upper layer is biomineralized with calcium phosphate (CaP) shell to add a pH responsive feature for increased sensitivity as well as protection from nonspecific reactions. This colorimetric sensor achieved minimally invasive extraction of the interstitial fluid from mice and converted glucose level to a visible color change promptly. Quantitative red green and blue (RGB) information could be obtained through a scanned image of the microneedle. This costless, portable colorimetric sensor could potentially detect daily glucose levels without blood drawing procedures.