PROPANEDIOL ALTERS INTRACELLULAR PH AND DEVELOPMENTAL POTENTIAL OF MOUSE ZYGOTES INDEPENDENTLY OF VOLUME CHANGE
PROPANEDIOL ALTERS INTRACELLULAR PH AND DEVELOPMENTAL POTENTIAL OF MOUSE ZYGOTES INDEPENDENTLY OF VOLUME CHANGE
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DOI：
10.1093/oxfordjournals.humrep.a137072
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发表时间：
1990-02-01
影响因子：
6.1
通讯作者：
PELUSO, JJ
中科院分区：
文献类型：
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作者：
DAMIEN, M;LUCIANO, AA;PELUSO, JJ
处理前，将小鼠受精卵与吖啶橙子（AO）（一种在生理pH范围内发荧光的荧光染料）一起孵育。在生理pH范围内取出受精卵。然后用1，2-丙二醇（PROH）以0.18mol/min、0.36mol/min和1.0mol/min的速率灌注受精卵，并监测受精卵体积和细胞内pH。用磷酸盐缓冲盐水灌注的受精卵保持其初始体积和AO荧光。以1 mol/min的速率暴露于PROH的所有受精卵的体积都减少，并且在10 min时失去了它们的AO荧光。以≤ 0.36 mol/min灌注的受精卵的体积没有改变。然而，在这方面，在0.18 mol/min时，95%的受精卵在10 min时保持其荧光，49%在15 min时保持其荧光。所有暴露于PROH的受精卵在20 min时都失去其荧光。3.0M PROH作用2.5min对细胞发育无影响，囊胚发育率低于对照组（P < 0.05）。长时间暴露导致2细胞和囊胚发育显著减少。这些数据表明暴露于≥ 2.5M PROH 2-7分钟改变了细胞内pH和发育潜力。由于这些重金属的影响是独立的体积变化，因此细胞内的PROH浓度，这是假定，PROH介导的毒性作用，直接改变细胞膜。
Prior to treatment, mouse zygotes were incubated with acridine orange (AO) a fluorescent dye which fluoresces within a physiological pH range. The zygotes were taken within a physiological pH range. The zygotes were then perifused with 1,2-propanediol (PROH) at rates of 0.18 mol/min, 0.36 mol/min and 1.0 mol/min and zygote volume and intracellular pH monitored. Zygotes perifused with phosphate-buffered saline maintained their initial volume and AO fluorescence. All of the zygotes exposed to PROH at a rate of 1 mol/min decreased in volume and lost their AO fluorescence by 10 min. The volume of the zygotes perifused at .ltoreq.0.36 mol/min was not altered. However, only 25% of the zygotes perifused at 0.36 mol/min maintained their fluorescence at 10 min and all lost their fluorescence by 15 min. At 0.18 mol/min 95% of the zygotes maintained their fluorescence at 10 min and 49% at 15 min. All of the PROH-exposed zygotes lost their fluorescence by 20 min. Although 2-cell development was not affected by 3.0 M PROH for 2.5 min, blastocyst development was reduced compared with controls (P < 0.05). Longer exposures resulted in a significant decrease in both 2-cell and blastocyst development. These data demonstrate that a 2-7-min exposure to .gtoreq.2.5 M PROH alters both the intracellular pH and developmental potential. Since these detrimetal effects are independent of volume changes and therefore intracellular PROH concentrations, it is postulated that PROH mediates its toxic action by directly altering the cell membrane.