Morphometric analysis of murine skin wound healing: Standardization of experimental procedures and impact of an advanced multitissue array technique
Morphometric analysis of murine skin wound healing: Standardization of experimental procedures and impact of an advanced multitissue array technique
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DOI：
10.1111/j.1524-475x.2006.00191.x
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发表时间：
2007-01-01
影响因子：
2.9
通讯作者：
Wickenhauser, Claudia
中科院分区：
文献类型：
--
作者：
Gerharz, Michael;Baranowsky, Ankev;Wickenhauser, Claudia
基于皮肤创伤的形态学数据为转基因小鼠模型的表型表征提供了重要信息。本研究的目的是比较不同小鼠品系中有关样品创伤、处理和评价的技术程序。多组织阵列技术被用来评估其适应性的标准化分析在伤口愈合。分析受伤后第1天和第14天之间的皮肤伤口。在94只小鼠中研究了小鼠品系（C57 BI/6 vs. FVB/N小鼠）、性别、穿孔活检大小和组织切片制备的影响。评估迁移舌（Δ MT）和未被上皮覆盖的表面之间的距离参数以描述上皮再生，并选择附件之间的距离来测量伤口收缩。此外，还对肉芽组织面积（GT）的测量技术进行了评价。这些数据说明了皮肤伤口愈合实验对标准化条件的要求，并证明连续切片的组织学制备对于检测伤口收缩的微小差异是强制性的。对于GT中的细胞组成的分析，多组织阵列是伤口愈合研究中有用的工具。
Morphometric data based on skin wounding offer important information for the characterization of the phenotype of transgenic mouse models. The goal of this study was the comparison of technical procedures concerning wounding, processing, and evaluation of samples in different mouse strains. The multitissue array technique was used to estimate its adaptability for standardized analysis in wound healing. Skin wounds between days 1 and 14 after wounding were analyzed. The influence of mouse strain (C57BI/6 vs. FVB/N mice), sex, size of the punch biopsies, and preparation of the tissue sections was investigated on 94 mice. The parameters distance between the migration tongues (delta MT) and surface not covered by epithelium were evaluated to describe the reepithelialization, and the distance between the adnexa was chosen to measure wound contraction. In addition, the techniques to measure the area of granulation tissue (GT) were evaluated. The data illustrate the requirement of standardized conditions for skin wound-healing experiments and demonstrate that histological preparation in serial sections is mandatory to detect slight differences in wound contraction. For the analysis of cellular composition in GT, multitissue arrays are useful tools in wound-healing studies.