Immunohistochemical renin study of DES-induced renal tumor in the Syrian hamster.
Immunohistochemical renin study of DES-induced renal tumor in the Syrian hamster.
复制标题
叙利亚仓鼠 DES 诱导肾肿瘤的免疫组织化学肾素研究。
DOI：
10.1002/aja.1001820406
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Inagami,T
中科院分区：
文献类型：
--
作者：
Dodge,AH;Brownfield,M;Reid,IA;Inagami,T
己烯雌酚(DES)治疗一只叙利亚雄性仓鼠，导致肾肿瘤及其广泛散布的阴囊转移。原发瘤和转移性结节的细胞均含有分泌颗粒。这些肿瘤被连续移植到DES支持和非DES支持的宿主仓鼠体内，直到出现DES非依赖性肿瘤。将兔抗小鼠唾液肾素抗血清和兔抗大鼠肾树脂抗血清分别与原发肿瘤、转移性结节和所有转移性肿瘤的切片反应。切片用PAP和VectorABC-AP法染色。所有肿瘤均可见肾素阳性物质。测定了携带肾肿瘤移植的宿主仓鼠的血浆肾素活性(PRA)，并与正常的未经DES处理的雄性和雌性仓鼠的PRA值进行了比较。结果发现，携带肿瘤移植瘤的宿主仓鼠的平均PRA值显著高于正常鼠。
Diethylstilbestrol (DES) treatment of a male Syrian hamster resulted in the development of a renal tumor and its widely scattered scrosal metastases. Cells in both the primary tumor and metastatic nodules contained secretory granules. The tumors were transplanted serially into DES‐supported and non‐DES‐supported host hamsters until DES‐independent tumors developed. Rabbit antiserum to mouse salivary renin and rabbit antiserum to rat kidney resin were reacted with sections of the primary tumor, metastatic nodules, and all transport tumors. The sections were stained by the PAP and Vector‐ABC‐AP procedures. Renin‐positive material was observed in all tumors. Plasma renin activity (PRA) was determined for the host hamsters carrying the renal tumor transplants and compared to the PRA values that had been determined for normal non‐DES‐treated male and female hamsters. It was found that the average PRA values of host hamsters carrying the tumor transplants were significantly higher than the normal PRA ralues.