The presence of benzene ring activating CoA ligases for aromatics degradation in the ANaerobic MEthanotrophic (ANME) archaea.
The presence of benzene ring activating CoA ligases for aromatics degradation in the ANaerobic MEthanotrophic (ANME) archaea.
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DOI：
10.1128/spectrum.01766-23
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发表时间：
2023-09-27
影响因子：
3.7
通讯作者：
Zhou, Ning-Yi
中科院分区：
文献类型：
--
作者：
Liu, Wei-Wei;Pan, Piaopiao;Zhou, Ning-Yi
冷泉环境中存在石油源和黑碳源芳香族化合物，其中厌氧甲烷氧化（ANME）古菌作为优势微生物群落介导甲烷厌氧氧化产生无机碳和有机碳。通过预测ANME宏基因组组装基因组中的芳香族化合物分解代谢途径，我们提供了基因组和生化证据，表明ANME具有通过CoA活化苯环的策略，使用苯乙酸和苯甲酸作为底物代谢芳香族化合物的潜力。两种环活化酶苯乙酸-CoA连接酶（PaaKANME）和苯甲酸-CoA连接酶（BadAANME）能够分别在体外将苯乙酸转化为苯乙酰-CoA和将苯甲酸转化为苯甲酰-CoA。它们嗜温、耐碱性，具有广泛的底物光谱，与各种底物表现出不同的亲和力。对ANME基因组和冷泉环境中相对基因丰度的探索表明，大约50%的ANME基因组含有PCL基因，并且各种细菌和古细菌含有PCL和BCL基因。这为ANME异养代谢芳香族化合物的能力提供了依据。这不仅增强了我们对ANME营养范围的理解，而且有助于探索这种无处不在的沉积古菌在冷渗漏环境中的碳流动中的额外生态和生物地球化学意义。厌氧甲烷氧化古菌是冷泉环境中甲烷厌氧氧化的主要微生物群落。因此推测ANME可能参与了芳香族化合物的代谢。在这里，我们提供的芳香族化合物的异养代谢ANME的基因组和生物化学证据，提高我们的营养范围的理解，也揭示了这些无处不在的沉积古菌在冷渗漏环境中的碳流动的生态和地球化学意义。总的来说，这项研究为ANME的代谢能力及其对全球碳循环的潜在贡献提供了有价值的见解。
Petroleum-source and black carbon-source aromatic compounds are present in the cold seep environments, where ANaerobic MEthanotrophic (ANME) archaea as the dominant microbial community mediates the anaerobic oxidation of methane to produce inorganic and organic carbon. Here, by predicting the aromatics catabolic pathways in ANME metagenome-assembled genomes, we provide genomic and biochemical evidences that ANME have the potential of metabolizing aromatics via the strategy of CoA activation of the benzene ring using phenylacetic acid and benzoate as the substrates. Two ring-activating enzymes phenylacetate-CoA ligase (PaaKANME) and benzoate-CoA ligase (BadAANME) are able to convert phenylacetate to phenylacetyl-CoA and benzoate to benzoyl-CoA in vitro, respectively. They are mesophilic, alkali resistance, and with broad substrate spectra showing different affinity with various substrates. An exploration of the relative gene abundance in ANME genomes and cold seep environments indicates that about 50% of ANME genomes contain PCL genes, and various bacteria and archaea contain PCL and BCL genes. The results provide evidences for the capability of heterotrophic metabolism of aromatic compounds by ANME. This has not only enhanced our understanding of the nutrient range of ANME but also helped to explore the additional ecological and biogeochemical significance of this ubiquitous sedimentary archaea in the carbon flow in the cold seep environments. ANaerobic MEthanotrophic (ANME) archaea is the dominant microbial community mediating the anaerobic oxidation of methane in the cold seep environments, where aromatic compounds are present. Then it is hypothesized that ANME may be involved in the metabolism of aromatics. Here, we provide genomic and biochemical evidences for the heterotrophic metabolism of aromatic compounds by ANME, enhancing our understanding of their nutrient range and also shedding light on the ecological and biogeochemical significance of these ubiquitous sedimentary archaea in carbon flow within cold seep environments. Overall, this study offers valuable insights into the metabolic capabilities of ANME and their potential contributions to the global carbon cycle.
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影响因子：
5.1
作者：
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通讯作者：
Boll, Matthias
DOI：
10.1073/pnas.0905073106
发表时间：
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影响因子：
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影响因子：
5.1
作者：
Liu, Wei-Wei;Pan, Jie;Zhou, Ning-Yi
通讯作者：
Zhou, Ning-Yi