Multi-electrode stimulation and recording in the isolated retina
Multi-electrode stimulation and recording in the isolated retina
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DOI：
10.1016/s0165-0270(00)00246-6
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发表时间：
2000-08-15
影响因子：
3
通讯作者：
Rizzo, JF
中科院分区：
文献类型：
--
作者：
Grumet, AE;Wyatt, JL;Rizzo, JF
作为视网膜外膜假体可行性探索的一部分，我们开发了一种实验方法，使用微制造的多电极阵列电刺激和记录视网膜神经元。将离体视网膜置于直径为10 μ m的圆盘电极阵列上，视网膜的神经节细胞侧面向电极表面。将视网膜浸泡在充氧的艾姆斯培养基中并加热，以使其在体外维持实验期间。通常4-9小时。为了减少刺激伪影，电极被分成两组-一组用于刺激，另一组用于记录-间隔数百微米，电极用氮化硅和10 μ m厚的聚酰亚胺层绝缘。使用光学隔离的电流源刺激器将刺激递送到阵列，并且用八通道神经响应放大器记录所产生的响应。刺激和记录在计算机控制下进行。描述了各种生理测量，以说明这种方法的优点和缺点。(C)2000 Elsevier Science B. V.保留所有权利。
As part of an exploration of the feasibility of an epi-retinal prosthesis, we developed an experimental method to electrically stimulate and record from retinal neurons using a micro-fabricated multi-electrode array. An isolated retina is placed on an array of 10 mu m diameter disk electrodes with the ganglion cell side of the retina facing the electrode surfaces. The retina is bathed in oxygenated Ames' medium and warmed in order to sustain it in vitro for the duration of an experiment. typically 4-9 h. To reduce stimulus artifacts, the electrodes are grouped into two clusters - one used for stimulation and the other for recording - spaced several hundred microns apart, and electrodes are insulated with both silicon nitride and a 10 mu m thick layer of polyimide. Stimuli are delivered to the array using an optically isolated current source stimulator, and the resulting responses recorded with an eight channel nerve response amplifier. Stimulation and recording are performed under computer control. A variety of physiologic measurements is described in order to illustrate the strengths and drawbacks of this method. (C) 2000 Elsevier Science B.V. All rights reserved.