PHYCOERYTHRIN FLUORESCENCE-BASED ASSAY FOR PEROXY-RADICALS - A SCREEN FOR BIOLOGICALLY RELEVANT PROTECTIVE AGENTS
PHYCOERYTHRIN FLUORESCENCE-BASED ASSAY FOR PEROXY-RADICALS - A SCREEN FOR BIOLOGICALLY RELEVANT PROTECTIVE AGENTS
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DOI：
10.1016/0003-2697(89)90056-0
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发表时间：
1989-03-01
影响因子：
2.9
通讯作者：
GLAZER, AN
中科院分区：
文献类型：
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作者：
DELANGE, RJ;GLAZER, AN
在该测定的条件下，快速与过氧自由基反应的抗氧化剂（例如，抗坏血酸盐、维生素E类似物、尿酸盐）完全保护藻红蛋白免受由2，2“-偶氮二（2-脒基丙烷）热分解产生的自由基的损害;其它化合物提供部分浓度依赖性保护。藻红蛋白荧光发射随时间的变化提供了自由基损伤速率的量度。该测定利用藻红蛋白对这些过氧自由基的不寻常的反应性。在摩尔基础上，藻红蛋白与这些自由基的反应比抗坏血酸盐或维生素E类似物慢100倍以上，但比其他蛋白质快60倍以上。本试验的应用，以估计人体血浆的过氧自由基清除能力的描述，并比较几种蛋白质和DNA，维生素及其衍生物，儿茶酚胺类神经递质，和各种其他低分子量的生物化合物的清除性能。
Under the conditions of this assay, antioxidants that react rapidly with peroxy free radicals (e.g., ascorbate, vitamin E analogs, urate), protect phycoerythrin completely from damage by such radicals generated by thermal decomposition of 2,2''-azobis(2-amidinopropane); other compounds provide partial concentration-dependent protection. Change in phycoerythrin fluorescence emission with time provides a measure of the rate of free radical damage. The assay exploits the unusual reactivity of phycoerythrin toward these peroxy radicals. On a molar basis, phycoerythrin reacts with these radicals over 100-fold slower than do ascorbate or vitamin E analogs, but over 60-fold faster than other proteins. Applications of this assay to the estimation of the peroxy radical scavenging capacity of human plasma are described, and to the comparison of the scavenging properties of several proteins and of DNA, of vitamins and their derivatives, of catecholamine neurotransmitters, and of a variety of other low molecular weight biological compounds.