Functionalised iron oxide nanoparticles for multimodal optoacoustic and magnetic resonance imaging
Functionalised iron oxide nanoparticles for multimodal optoacoustic and magnetic resonance imaging
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DOI：
10.1039/c8tb02299b
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发表时间：
2019-04-07
影响因子：
7
通讯作者：
Parkin, Ivan P.
中科院分区：
文献类型：
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作者：
Bell, Gavin;Balasundaram, Ghayathri;Parkin, Ivan P.
光声(OA)分子吲哚青绿(ICG)和弗拉玛(R)774新型附着在氧化铁(Fe3O4)纳米颗粒的核心上，使多模式成像探针的合成变得简单，可用于多光谱光声断层成像(MSOT)和磁共振成像(MRI)。对纳米粒子进行了结构、光学和磁学分析，证明了其多峰特性。对染料ICG和Flamma(R)774的分析表明，当它们连接到氧化铁核心上时，它们在近红外波长分别有790和780 nm的吸收。这些波长是从任何内源性对比度中分离探测器强度的理想波长，例如氧离子(HBO(2))和脱氧血红蛋白(Hb)。MRI显示柠檬酸铁帽的R2对比度为230 mm(-1)，S为(-1)，与文献值一致。经光声染料修饰后，R2弛豫系数与Flamma(R)774氧化铁纳米颗粒(FeO-774)相当，R2=212 mm(-1)S(-1)，这表明OA染料附着物对磁共振对比度几乎没有影响。吲哚青绿功能化氧化铁(FeO-ICG)纳米颗粒的R2对比度在R2=5 mm(-1)S(-1)时显著降低。这些结果表明，利用氧化铁核心和简单的配体配位化学以及使用光声染料可以方便地合成有效的双模式MRI-MSOT探针。
The novel attachment of the optoacoustic (OA) molecules indocyanine green (ICG) and Flamma (R) 774 to the core of an iron oxide (Fe3O4) nanoparticle has resulted in the facile synthesis of a multimodal imaging probe for both multispectral optoacoustic tomography (MSOT) imaging and magnetic resonance imaging (MRI). The nanoparticles have been analysed structurally, optically and magnetically to demonstrate the multimodal characteristics. The OA analysis of the dyes ICG and Flamma (R) 774 showed that they have absorbance at the near IR wavelengths of 790 and 780 nm, respectively, when conjugated to an iron oxide core. These wavelengths are ideal for spectral unmixing of the probe intensity from any endogenous contrast, such as oxy-(HbO(2)) and deoxy-hemoglobin (Hb). MRI showed that citrate capped Fe3O4 exhibited a good r2 contrast of 230 mM(-1) s(-1), which is in line with literature values. Upon optoacoustic dye modification, the r2 relaxivity coefficient is comparable with that of Flamma (R) 774 iron oxide nanoparticles (FeO-774) with r2 = 212 mM(-1) s(-1), showing that an OA dye attachment can have little to no effect on the MRI contrast. Indocyanine green functionalised iron oxide (FeO-ICG) nanoparticles showed an r2 contrast that was dramatically reduced with r2 = 5 mM(-1) s(-1). These results indicate that the facile synthesis of an effective dual modality MRI-MSOT probe can be developed using an iron oxide core and simple ligand coordination chemistry using an optoacoustic dye.