Efficiency of Semen Cryopreservation in Stallions
Efficiency of Semen Cryopreservation in Stallions
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DOI：
10.3390/ani10061033
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发表时间：
2020-06-01
期刊：
影响因子：
3
通讯作者：
Aurich, Christine
中科院分区：
文献类型：
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作者：
Aurich, Joerg;Kuhl, Juliane;Aurich, Christine
不同种马冷冻和解冻后的精液质量不同，但这种差异是否也受到马品种的影响尚不清楚。本研究回顾性分析了来自5个品种134种马的1012份冻融射精的精液质量。然后计算人工授精可接受的射精百分比和每次射精的授精剂量。冷冻前的精子活力是人工智能可接受的解冻后射精质量百分比的最重要解释变量。在研究的其他变量中，种马年龄是最重要的参数，在9岁以上的种马中，可接受射精的百分比明显下降。与温血马、四分之一马和冰岛马相比，阿拉伯种马更容易接受冻融射精。因此，分析表明，在可接受的冷冻保存的射精品种之间的百分比的差异。精子总数是决定每次可接受的冻融射精所获得的精液剂量的最重要变量。不同种马的精子的低温耐受性存在差异，但这种差异在多大程度上受品种的影响还有待确定。本研究回顾性分析了5个品种134头种马1012次精液冷冻保存后的精液质量。计算可用于人工授精（AI）的冻融射精的百分比和每个冷冻保存的射精的授精剂量。逻辑回归分析显示，原始精液中的精子活力是冷冻保存的精液解冻后质量为人工智能可接受的百分比的最重要的解释变量。在模型中包含的其他变量中，种马年龄是最重要的参数，年轻的种马比年长的种马更容易接受射精。Logistic回归也显示阿拉伯种马比温血马、四分之一马和冰岛马更容易接受冻融射精。因此，分析表明，在可接受的冷冻保存的射精百分比的马品种之间的差异。季节是一个不太相关的解释变量的百分比可接受的冷冻保存射精。逻辑回归显示，精子总数是决定每次可接受射精获得的冷冻保存精液剂量数量的最重要变量。总之，逻辑回归分析显示，种马年龄和品种是人工智能可接受的冷冻保存射精百分比的解释变量。
Simple Summary The quality of stallion semen after freezing and thawing differs among stallions, but it remains to be determined whether such differences are also affected by horse breed. In this study, semen quality was analysed retrospectively in 1012 frozen-thawed ejaculates from 134 stallions of 5 breeds. The percentage of ejaculates acceptable for artificial insemination (AI) and the number of insemination doses per ejaculate was then calculated. Sperm motility before freezing was the most important explanatory variable for the percentage of ejaculates with a post-thaw quality acceptable for AI. Of the other variables studied, stallion age was the most important parameter and a decrease in the percentage of acceptable ejaculates was evident in stallions older than 9 years. There were also more acceptable frozen-thawed ejaculates in Arab stallions compared to Warmbloods, Quarter Horses and Icelandic horses. The analysis thus demonstrates differences in the percentage of acceptable cryopreserved ejaculates among breeds. Total sperm count was the most important variable determining the number of semen doses obtained per acceptable frozen-thawed ejaculate. Differences in the cryotolerance of spermatozoa exist among stallions, but it remains to be determined to what extent such differences are affected by breed. In this study, post-thaw semen quality in stallions presented for semen cryopreservation was analysed retrospectively (1012 ejaculates from 134 stallions of 5 breeds). The percentage of frozen-thawed ejaculates acceptable for artificial insemination (AI) and the number of insemination doses per cryopreserved ejaculate was calculated. Logistic regression analysis revealed sperm motility in raw semen as the most important explanatory variable for the percentage of cryopreserved ejaculates with a post-thaw quality acceptable for AI. Of the other variables included into the model, stallion age was the most important parameter with more acceptable ejaculates in younger than in older stallions. Logistic regression also showed more acceptable frozen-thawed ejaculates in Arab stallions versus Warmbloods, Quarter Horses and Icelandic horses. The analysis thus demonstrates differences in the percentage of acceptable cryopreserved ejaculates among horse breeds. Season was a less relevant explanatory variable for percentage of acceptable cryopreserved ejaculates. Logistic regression revealed total sperm count as the most important variable determining the number of cryopreserved semen doses obtained per acceptable ejaculate. In conclusion, logistic regression analysis revealed stallion age and breed as explanatory variables for the percentage of cryopreserved ejaculates acceptable for AI.