Essential role of Toll-like receptors for dendritic cell and NK1.1+ cell-dependent activation of type 1 immunity by Lactobacillus pentosus strain S-PT84
Essential role of Toll-like receptors for dendritic cell and NK1.1+ cell-dependent activation of type 1 immunity by Lactobacillus pentosus strain S-PT84
复制标题
DOI：
10.1016/j.imlet.2008.06.003
复制
发表时间：
2008-09-30
影响因子：
4.4
通讯作者：
Nishimura, Takashi
中科院分区：
文献类型：
--
作者：
Koizumi, Shin-ichi;Wakita, Daiko;Nishimura, Takashi
1型免疫的激活在宿主抵抗传染病和肿瘤的防御机制中起着至关重要的作用。存在于胃肠道中的乳酸菌是诱导1型优势免疫的有力工具之一，可能改善th2依赖性过敏性疾病。在本研究中，我们发现小鼠口服戊糖乳杆菌S-PT84菌株可显著提高脾细胞NK活性。我们进一步发现NK1.1阳性NK细胞和NKT细胞是体外S-PT84刺激后产生ifn - γ的细胞。S-PT84通过CD11c(+) dc以toll样受体（TLR） 2-和/或tlr4依赖的方式激活IL-12的产生，诱导ifn - γ产生细胞。有趣的是，DCs和NK1.1（+）细胞之间的直接相互作用也是S-PT84刺激下ifn - γ产生的必要条件。因此，我们得出结论，S-PT84通过dc和NK1.1（+）细胞分别产生的IL-12和ifn - γ有效促进I型免疫。因此，S-PT84可能是一种很好的改善免疫平衡的免疫调节剂，在控制过敏、感染性疾病和肿瘤等方面发挥着关键作用。(C) 2008 Elsevier B.V.版权所有
Activation of type 1 immunity plays a critical role in host defense mechanisms against infectious disease and tumor. Lactic acid bacteria, existing in the gastrointestinal tract, are one of the powerful tools to induce a type-1-dominant immunity, which may improve Th2-dependent allergic diseases. In the present work, we found that an oral intake of Lactobacillus pentosus strain, S-PT84 into mice significantly enhanced NK activity of spleen cells in vivo. We further revealed that NK1.1 positive NK cells and NKT cells are responsible cells for producing IFN-gamma after stimulation with S-PT84 in vitro. S-PT84 induced IFN-gamma-producing cells through activation of IL-12 production by CD11c(+)DCs in Toll-like receptor(TLR) 2- and/or TLR4-dependent manner. Interestingly, direct interaction between DCs and NK1.1(+) cells was also essential for the IFN-gamma production in response to the S-PT84 stimulation. Therefore, we concluded that S-PT84 effectively promoted type I immunity through IL-12 and IFN-gamma which were produced by DCs and NK1.1(+) cells, respectively. Thus, S-PT84 would be a nice immune modulator for improving immunobalance, which plays a pivotal role for controlling allergy, infectious diseases and tumor. (C) 2008 Elsevier B.V. All rights reserved.