Intectin, a novel small intestine-specific glycosylphosphatidylinositol-anchored protein, accelerates apoptosis of intestinal epithelial cells
Intectin, a novel small intestine-specific glycosylphosphatidylinositol-anchored protein, accelerates apoptosis of intestinal epithelial cells
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DOI：
10.1074/jbc.m408047200
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发表时间：
2004-10-08
影响因子：
4.8
通讯作者：
Shimomuraa, I
中科院分区：
文献类型：
--
作者：
Kitazawa, H;Nishihara, T;Shimomuraa, I
肠上皮细胞经历快速更新和脱落，特别是在绒毛尖端。这个过程受到各种营养素的调节，尤其是脂肪。细胞凋亡是这种周转的重要调节机制之一。因此，确定控制上皮细胞凋亡的因素有助于我们了解肠粘膜更新的机制。在这里，我们报告了一个新的小的精氨酸特异性成员的Ly-6家庭，intectin，信号序列陷阱方法的识别。Intectin mRNA表达仅在肠中被鉴定，并且定位于肠粘膜的绒毛尖端，已知其经历细胞凋亡。Intectin mRNA表达受营养调控。表达intectin的肠上皮细胞对棕榈酸诱导的细胞凋亡更敏感，与对照组相比，这种作用伴随着caspase-3活性的增加。Intectin表达也降低了肠上皮细胞的细胞-细胞粘附。
Intestinal epithelial cells undergo rapid turnover and exfoliation especially at the villus tips. This process is modulated by various nutrients especially fat. Apoptosis is one of the important regulatory mechanisms of this turnover. Therefore, identification of the factors that control epithelial cell apoptosis should help us understand the mechanism of intestinal mucosal turnover. Here, we report the identification of a novel small intestine-specific member of the Ly-6 family, intectin, by signal sequence trap method. Intectin mRNA expression was exclusively identified in the intestine and localized at the villus tips of intestinal mucosa, which is known to undergo apoptosis. Intectin mRNA expression was modulated by nutrition. Intestinal epithelial cells expressing intectin were more sensitive to palmitate-induced apoptosis, compared with control intestinal epithelial cells, and such effect was accompanied by increased activity of caspase-3. Intectin expression also reduced cell-cell adhesion of intestinal epithelial cells.