Immunochemical characterization of the mucoid exopolysaccharide of Pseudomonas aeruginosa.
Immunochemical characterization of the mucoid exopolysaccharide of Pseudomonas aeruginosa.
复制标题
铜绿假单胞菌粘液胞外多糖的免疫化学特征。
DOI：
10.1093/infdis/147.3.494
复制
发表时间：
1983
期刊：
影响因子：
--
通讯作者：
Eardley,DD
中科院分区：
文献类型：
--
作者：
Pier,GB;MatthewsJr,WJ;Eardley,DD
从三株产气假单胞菌中分离出藻酸样粘多糖。纯化的类粘抗原为>99%糖醛酸。通过血凝试验，在用完整的粘液生物体或纯化的粘液外泌多糖免疫家兔后，记录了对粘液外泌多糖的抗体应答。来自一个菌株（编号2192）的粘液抗原主要由来自不同临床分离株的40种藻酸盐胞外多糖共享的单一血清学表位组成。来自其他两种菌株的粘液样外泌多糖（nos. 1和258）除了共同表位之外还具有表型特异性决定簇。正常成人、儿童和囊性纤维化患者血清中粘液P培养阳性和阴性抗体分析嗜水气单胞菌血凝滴度升高与粘液P培养阳性之间有高度显著性相关（P<0.001）。铜绿。
Alginic acid-like mucoid exopolysaccharide was isolated from three strains ofPseudomonas aeruginosaobtained from the sputa of patients with cystic fibrosis. Purified mucoid antigens were >99% uronic acid. With a hemagglutination assay, antibody responses to the mucoid exopolysaccharide were documented after immunization of rabbits with either whole mucoid organisms or purified mucoid exopolysaccharide. The mucoid antigen from one strain (no. 2192) was composed predominantly of a single serologic epitope shared among 40 alginate exopolysaccharides from different clinical isolates. The mucoid exopolysaccharide from the other two strains (nos. 1 and 258) had a serotype-specific determinant in addition to the common epitope. Analyses of antibody in sera from normal adults, children, and patients with cystic fibrosis culturepositive and culture-negative for mucoidP. aeruginosashowed a highly significant (P<0.001) association between increased hemagglutination titers and positive cultures for mucoidP. aeruginosa.