Immunocytochemical localization of glutamate-, glutaminase- and aspartate aminotransferase-like immunoreactivity in the rat deep cerebellar nuclei.
Immunocytochemical localization of glutamate-, glutaminase- and aspartate aminotransferase-like immunoreactivity in the rat deep cerebellar nuclei.
复制标题
大鼠小脑深部核中谷氨酸、谷氨酰胺酶和天冬氨酸转氨酶样免疫反应性的免疫细胞化学定位。
DOI：
10.1016/0006-8993(86)91024-3
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发表时间：
1986
期刊：
影响因子：
2.9
通讯作者：
Mullett,MA
中科院分区：
文献类型：
--
作者：
Monaghan,PL;Beitz,AJ;Larson,AA;Altschuler,RA;Madl,JE;Mullett,MA
虽然小脑深部核团的解剖结构和神经元之间的连接已经有了很好的研究，但对小脑传出神经通路中的神经递质系统却知之甚少。本研究利用免疫组织化学方法结合一种新的单克隆抗体特异性的谷氨酰胺固定的谷氨酸和多克隆抗血清对谷氨酰胺酶（GLNase）和天冬氨酸转氨酶（AATase）检查存在的假定兴奋性氨基酸递质的小脑深核神经元。在小脑外侧核、后间位核、前间位核和内侧深核的神经元中观察到了碳二亚胺固定的谷氨酸样、GLNase样和AAT酶样免疫反应。AAT酶抗血清染色神经元的数量多于GLN酶抗血清和单克隆抗体。这些结果表明，谷氨酸，GLNA酶和AAT酶存在于小脑深核的神经元，并提出谷氨酸可能是这些结构中的兴奋性递质的可能性。
Althougthe anatomy and the connectivity of the deep cerebellar nuclei have been well documented, little is known about the neurotransmitter systems mediating cerebellar efferent pathways. The present study utilizes immunohistochemical procedures in conjunction wita novel monoclonal antibody specific for carbodiimide-fixed glutamate and polyclonal antisera against glutaminase (GLNase) and aspartate aminotransferase (AATase) to examine the presence of putative excitatory amino acid transmitters in neurons of the deep cerebellar nuclei. Carbodiimide-fixed glutamate-like, GLNase-like and AATase-like immunoreactivities were observed in neurons of the lateral, posterior interpositus, anterior interpositus and medial deep cerebellar nuclei. More neurons were stained witAATase antiserum than witthe GLNase antiserum or the monoclonal antibody. These results suggest glutamate, GLNase and AATase are present in neurons of the deep cerebellar nuclei and raise the possibility that glutamate may be an excitatory transmitter in these structures.