Smoking a single cigarette rapidly reduces combined concentrations of nitrate and nitrite and concentrations of antioxidants in plasma
Smoking a single cigarette rapidly reduces combined concentrations of nitrate and nitrite and concentrations of antioxidants in plasma
复制标题
DOI：
10.1161/hc1002.105935
复制
发表时间：
2002-03-12
期刊：
影响因子：
37.8
通讯作者：
Inoue, M
中科院分区：
文献类型：
--
作者：
Tsuchiya, M;Asada, A;Inoue, M
背景-吸烟是众所周知的心血管疾病发展的危险因素，但其作用机制尚不完全清楚。由于香烟烟雾中含有超氧化物和其他活性氧物种，人们推测吸烟的一些不良影响可能是由于内皮细胞的氧化损伤，从而导致一氧化氮(NO)的短缺。然而，关于吸烟对血浆NO和抗氧化剂浓度的急性影响的信息很少。我们测量了吸烟者吸烟后血浆硝酸盐和亚硝酸盐浓度的变化，以及主要血清抗氧化剂(抗坏血酸、半胱氨酸、蛋氨酸和尿酸)浓度的变化，作为NO浓度的指标。方法和结果-对20名吸烟者进行了随机交叉研究。抽假烟不会引起所有检测参数的显著变化。然而，与吸烟前和假吸烟时相比，吸一支香烟显著降低了血浆中硝酸盐和亚硝酸盐的浓度，分别降低了3.5+/-1.2和3.4+/-1.1摩尔/L。吸一支香烟后，抗坏血酸和其他抗氧化剂的浓度也显著降低。这些参数在戒烟60分钟后恢复到实验前的水平。结论--目前的发现表明，吸烟一支香烟会暂时降低血浆中硝酸盐、亚硝酸盐和血清抗氧化剂的浓度。这些短暂的变化可能部分导致了吸烟后经常观察到的冠状动脉血管收缩。
Background-Cigarette smoking is a well-known risk factor for the development of cardiovascular disease, yet the mechanism of action involved is not completely understood. Because cigarette smoke contains superoxide and other reactive oxygen species, it has been hypothesized that some of the adverse effects of smoking may result from oxidative damage to endothelial cells, which results in nitric oxide (NO) shortage. However, little information is available regarding the acute effects of smoking on plasma concentrations of NO and antioxidants. We measured the changes in the combined plasma concentrations of nitrate and nitrite as an index of NO concentration, as well as changes in concentrations of major serum antioxidants (ascorbic acid, cysteine, methionine, and uric acid) in smokers after smoking a single cigarette.Methods and Results-A randomized crossover study of the effects of smoking a single cigarette was performed in 20 smokers. Smoking a sham cigarette induced no significant changes in all assayed parameters. However, smoking a single cigarette significantly decreased nitrate and nitrite plasma concentrations by 3.5+/-1.2 and 3.4+/-1.1 mumol/L, compared with plasma concentrations at presmoking and sham smoking, respectively. The concentrations of ascorbic acid and other antioxidants were also significantly lower after smoking a single cigarette. These parameters returned to preexperimental levels 60 minutes after smoking cessation.Conclusion-The present findings indicate that smoking a single cigarette temporarily decreases nitrate, nitrite, and serum antioxidant concentrations in the plasma. These transient changes may partially contribute to coronary vasoconstriction, which is routinely observed after smoking.