Uptake of Noncytotoxic Acid-Treated Single-Walled Carbon Nanotubes into the Cytoplasm of Human Macrophage Cells
Uptake of Noncytotoxic Acid-Treated Single-Walled Carbon Nanotubes into the Cytoplasm of Human Macrophage Cells
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DOI：
10.1021/nn900416z
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发表时间：
2009-06-01
期刊：
影响因子：
17.1
通讯作者：
Welland, Mark
中科院分区：
文献类型：
--
作者：
Porter, Alexandra E.;Gass, Mhairi;Welland, Mark
水溶性单壁纳米管（SWNTs）正在被测试作为医学成像和治疗活性分子到靶细胞的传递的造影剂。然而，在它们商业化使用之前，必须确定它们的亚细胞分布以及它们是否具有细胞毒性。在这里，我们的特点吸收未标记的，酸处理，水溶性单壁碳纳米管的人单核细胞衍生的巨噬细胞使用组合的拉曼光谱和分析电子显微镜，并比较我们的研究结果，以前的工作未纯化的单壁碳纳米管。拉曼光谱表明，酸处理的单壁碳纳米管的碳壁上的官能团比未经处理的单壁碳纳米管的数量更多。酸处理的单壁碳纳米管比未纯化的单壁碳纳米管在细胞内较少聚集。束，也是个人的酸处理的单壁碳纳米管，经常被发现内溶酶体和细胞质，在那里他们没有引起显着的变化，细胞活力或结构，即使在4天的曝光。
Water-soluble single-walled nanotubes (SWNTs) are being tested as contrast agents for medical imaging and for the delivery of therapeutically active molecules to target cells. However, before they become used commercially, it will be essential to establish their subcellular distribution and whether they are cytotoxic. Here we characterize uptake of unlabeled, acid-treated, water-soluble SWNTs by human monocyte derived macrophage cells using a combination of Raman spectroscopy and analytical electron microscopy and compare our findings to previous work on unpurified SWNTs. Raman spectroscopy demonstrated that acid-treated SWNTs had a greater number of functional groups on the carbon walls than nontreated SWNT. The acid-treated SWNTs were less aggregated within cells than unpurifed SWNTs. Bundles, and also individual acid-treated SWNTs, were found frequently inside lysosomes and also the cytoplasm, where they caused no significant changes in cell viability or structure even after 4 days of exposure.