AGS cell line xenograft tumor as a suitable gastric adenocarcinoma model: growth kinetic characterization and immunohistochemistry analysis.
AGS cell line xenograft tumor as a suitable gastric adenocarcinoma model: growth kinetic characterization and immunohistochemistry analysis.
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DOI：
10.22038/ijbms.2018.22938.5835
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发表时间：
2018-07
影响因子：
2.2
通讯作者：
Amanpour S
中科院分区：
文献类型：
--
作者：
Barati T;Haddadi M;Sadeghi F;Muhammadnejad S;Muhammadnejad A;Heidarian R;Arjomandnejad M;Amanpour S
胃癌是全球第三大癌症相关死亡原因。病人的整体存活率很低，因为胃癌通常在晚期才被诊断出来。因此，需要进一步的研究和适当的研究工具来开发新的治疗方法。本研究中使用了8只具有C57 BL/6背景的雌性无胸腺裸鼠。将AGS细胞接种到侧腹中。计算肿瘤体积并绘制生长曲线。当肿瘤体积达到1000 mm 3时，用CO2气体人道处死动物。收获后，用苏木精和伊红（H&E）和免疫组织化学（IHC）分析肿瘤。病理学家通过H&E染色确认肿瘤实体。采用免疫组化法检测HER-2、P53、Ki-67、CD 34、细胞角蛋白8（CK 8）、波形蛋白、雌激素受体（ER）和孕酮受体（PR）的表达。平均倍增时间为40.984 d，潜伏期为30.62 d。H&E染色结果显示高度恶性的高染色质上皮细胞。免疫组化检测显示P53基因突变状态。肿瘤细胞中CK 8蛋白的表达评分为+3。波形蛋白不表达，未观察到间充质表型的变化。Ki-67免疫组化显示增殖率>43%，高MVD定义为血管生成。相应的AGS异种移植模型提供了一个机会，以了解肿瘤生长的模式，以及在体内研究中评价新的胃癌疗法。
Gastric cancer is the third leading cause of cancer-related death worldwide. The overall survival rate of patients is poor because gastric cancers are usually diagnosed at the late stages. Therefore, further research is needed and appropriate research tools are required to develop novel therapeutic approaches. Eight female athymic nude mice with a C57BL/6 background were used in this study. AGS cells were inoculated into the flank. The tumor volumes were calculated and growth curves were drawn. When the volume of the tumors reached 1000 mm3, the animals were humanely euthanized with CO2 gas. After harvesting, tumors were analyzed with Hematoxylin and Eosin (H&E) and immunohistochemistry (IHC). A pathologist confirmed tumor entity through H&E staining. Tumors were evaluated for expression of HER-2, P53, Ki-67, CD34, cytokeratin 8 (CK8), vimentin, estrogen receptor (ER), and progesterone receptor (PR) utilizing immunohistochemistry. The tumor take rate was 62.5%, mean doubling time was 40.984 d, and the latency period was 30.62 days. The H&E staining results showed highly malignant hyperchromatin epithelial cells. IHC assessment showed the mutation status of P53 gene. The expression score of the CK8 protein in the tumor cells was +3. Vimentin protein was not expressed and changes in mesenchymal phenotype were not observed. Ki-67 IHC indicated that the proliferation rate was >43% and angiogenesis was defined as high MVD. The respective AGS xenograft model provides an opportunity to understand the pattern of tumor growth as well as to evaluate new gastric cancer therapies in in vivo studies.