Acute and subacute toxicity studies of Pluronic P85/poly (lactic acid) nanoparticles in mice
Acute and subacute toxicity studies of Pluronic P85/poly (lactic acid) nanoparticles in mice
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DOI：
10.1049/mnl.2013.0461
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发表时间：
2013-12
影响因子：
1.3
通讯作者：
L. Yuping;Li‐Zhen Sun;Li-Hua Yao;X. Xiong;Zi-ling Li
中科院分区：
文献类型：
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作者：
L. Yuping;Li‐Zhen Sun;Li-Hua Yao;X. Xiong;Zi-ling Li
本研究的目的是评估聚乳酸-b- pluronic -b-聚乳酸（PLA-P85-PLA）纳米颗粒（NPs）的口服毒性，以证明其在给药应用中的适用性。在急性口服毒性研究中，以0.4 ml不同浓度（低浓度：20 mg/l，中浓度：100 mg/l，高浓度：500 mg/l）的PLA-P85-PLA NPs溶液喂养动物14 d；即使在高浓度治疗中也未观察到死亡或治疗相关并发症。在亚急性口服毒性试验中，NPs被口服给药28天。在研究结束时，采集血液样本进行生化分析。为了进行组织病理学分析，对动物的器官进行称重和处理。所有动物在研究期间均存活，临床体征、体重、饲料消耗量、生化指标、器官重量和组织病理学检查无明显变化。这些结果表明，口服给药后，PLA-P85-PLA NPs对小鼠没有产生与治疗相关的毒性，因此，它们可以用于潜在的治疗应用。
The objective of the present reported study was to evaluate the oral toxicity of poly (lactic acid)-b-Pluronic-b-poly (lactic acid) (PLA-P85-PLA) nanoparticles (NPs) so as to demonstrate their applicability for drug delivery applications. In acute oral toxicity studies, the animals were fed with 0.4 ml PLA-P85-PLA NPs solution in different concentrations (low concentration: 20 mg/l, intermediate concentration: 100 mg/l and higher concentration: 500 mg/l) for 14 days; no deaths or treatment related complications were observed even in the higher concentration treatment. In case of the subacute oral toxicity test, the NPs were administered orally to mice for a period of 28 days. At the end of the study, blood samples were collected for biochemical analysis. For histopathological analysis, organs of the animals were weighed and processed. All the animals survived during the study, with no significant changes in clinical signs, body weight, feed consumption, biochemistry parameters, organ weights and histopathological findings. These results demonstrate that PLA-P85-PLA NPs produced no treatment related toxicity in mice following oral administration, thus, they can be exploited for potential therapeutic applications.