Bacillus thuringiensis CrylAc Resistance Frequency in Tobacco Budworm (Lepidoptera: Noctuidae)
Bacillus thuringiensis CrylAc Resistance Frequency in Tobacco Budworm (Lepidoptera: Noctuidae)
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DOI：
10.1603/029.102.0149
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发表时间：
2009-02-01
影响因子：
2.2
通讯作者：
Azuara-Dominguez, Ausencio
中科院分区：
文献类型：
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作者：
Blanco, Carlos A.;Andow, David A.;Azuara-Dominguez, Ausencio
烟芽夜蛾Heliothis virescens（F.）（鳞翅目：夜蛾科），是棉花，陆地棉L.，对多种合成杀虫剂产生了抗药性表达CrylAc的棉花自引入北美以来已证明其对这种昆虫的有效性为19%。为了估计田间采集的烟草夜蛾对1.0 μ g苏云金芽孢杆菌CrylAc诊断浓度产生抗性的等位基因频率，对来自7个地理区域的1，001个单对家系的第二代（F-2）进行了研究，2006年和2007年从自然群体中筛选了202个等位基因，没有发现主要的抗性等位基因。新生儿的56个单对家庭能够发展到二龄的诊断浓度在初始屏幕上，但只有7个这些线这样做，再次在第二次验证性屏幕。对CrylAc的弱抗性等位基因可能在H.绿色的我们估计的抗性等位基因频率（0.0036-0.0263）与1993年发表的估计值没有显著差异。没有证据表明H。virescens种群变得对CrylAc更有抗性。
The tobacco budworm, Heliothis virescens (F.) (Lepidoptera: Noctuidae), is one of the most important: pests of cotton, Gossypium hirsutum L., that has become resistant to a wide range of synthetic insecticides. CrylAc-expressing cotton has proven its effectiveness against this insect since its introduction in North America in 19%. However, the constant exposure of tobacco budworm to this protein toxin may result in the development of resistance to it. To estimate the frequency of alleles that confer resistance to a 1.0 mu g of Bacillus thuringiensis CrylAc diagnostic concentration in field-collected insects, the second generation (F-2) of 1,001 single-pair families from seven geographical regions representing 2,202 alleles from natural populations was screened in 2006 and 2007 without finding major resistant alleles. Neonates of 56 single-pair families were able to develop to second instar on the diagnostic concentration in the initial screen, but only seven of these lines did so again in a second confirmatory screen. Minor resistance alleles to CrylAc may be quite common in natural populations of H. virescens. Our estimated resistance allele frequencies (0.0036-0.0263) were not significantly different from a previously published estimate from 1993. There is no evidence that H. virescens populations have become more resistant to CrylAc.