Semaphorin D acts as a repulsive factor for entorhinal and hippocampal neurons
Semaphorin D acts as a repulsive factor for entorhinal and hippocampal neurons
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Semaphorin D 充当内嗅和海马神经元的排斥因子
DOI：
10.1046/j.1460-9568.1999.00517.x
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发表时间：
1999
影响因子：
3.4
通讯作者：
T. Skutella
中科院分区：
文献类型：
--
作者：
A. Steup;O. Ninnemann;N. Savaskan;Robert Nitsch andreas W. Püschel;T. Skutella
我们分析了脑信号蛋白D对发育中大鼠内鼻-海马结构轴突的影响。从不同发育阶段获得内嗅皮层和海马原基的浅层外植体，并与表达脑信号蛋白D的细胞聚集体共培养。从早期胚胎阶段（E16和E17）分离的内嗅外植体延伸的神经突不受信号蛋白D的影响，但在后期阶段（E20和E21）被排斥。海马神经元轴突在E21时也被semaphorin D排斥。原位杂交研究显示，脑信号蛋白D受体neuropilin-1在E17-P7期的内嗅皮层中表达，在E17-成年期的齿状回和CA 1 -3区域中表达。这些数据表明，semaphorin D参与了穿孔通路的形成，并通过neuropilin-1受体作为排斥信号，阻止内嗅纤维生长到齿状回的颗粒层中。这些数据也表明了脑信号蛋白D在海马内连接的发展中的作用。
We analysed the effects of semaphorin D on axons from the developing rat entorhinal–hippocampal formation. Explants from superficial layers of the entorhinal cortex and of the hippocampus anlage were obtained from various developmental stages and co‐cultured with cell aggregates expressing semaphorin D. Neurites extending from entorhinal explants that had been isolated from early embryonic stages (E16 and E17) were not affected by semaphorin D, but were repelled at later stages (E20 and E21). Axons from hippocampal neurons explanted at E21 were also repelled by semaphorin D. In situ hybridization studies revealed expression of the semaphorin D receptor neuropilin‐1 in the entorhinal cortex from stage E17 to stage P7, and in the dentate gyrus and CA1–3 regions between E17 and adulthood. These data suggest that semaphorin D is involved in the formation of the perforant pathway and acts, via the neuropilin‐1 receptor, as a repulsive signal that prevents entorhinal fibres from growing into the granular layer of the dentate gyrus. These data also suggest a role for semaphorin D in the development of intrahippocampal connections.
腹侧脊髓外植体分泌的感觉轴突驱避剂被针对 collapsin-1 的抗体中和。
DOI：
10.1242/dev.124.7.1377
发表时间：
1997
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Shepherd,IT;Luo,Y;Lefcort,F;Reichardt,LF;Raper,JA
通讯作者：
Raper,JA