Protein corona components of polyethylene glycol-conjugated organosilica nanoparticles modulates macrophage uptake
Protein corona components of polyethylene glycol-conjugated organosilica nanoparticles modulates macrophage uptake
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DOI：
10.1016/j.colsurfb.2020.111527
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发表时间：
2021-03-01
影响因子：
5.8
通讯作者：
Nakamura, Michihiro
中科院分区：
文献类型：
--
作者：
Kim, Hyungjin;Roth, Daniel;Nakamura, Michihiro
制备了与不同分子量（2 K、12 K、20 K 和 30 K）的聚乙二醇 (PEG) 缀合的荧光有机二氧化硅纳米颗粒 (FNP)，以研究鼠源性巨噬细胞对它们的细胞摄取。在含有 FBS 的培养基中，与不含 FBS 的培养基相比，FNP-PEG 的细胞摄取减少，表明 FNP-PEG 上的蛋白冠减少了细胞摄取。牛血清白蛋白 (BSA) 和血红蛋白 (Hb) 被检测为所有 FNP-PEG 上最丰富的成分。用 BSA 和 Hb 预包被 FNP-PEG 会减少不含 FBS 的培养基中巨噬细胞的摄取，表明这些成分可能会增强 PEG 的隐形功能。此外，与不含 FBS 的培养基相比，含有 FBS 的培养基中 BSA 和 Hb 包被的 FNP-PEG 的摄取减少更多。 BSA和Hb可能是FNP-PEGs的体外隐形增强蛋白。
Fluorescent organosilica nanoparticles (FNP) conjugated with polyethylene glycol (PEG) of variant molecular weight (2 K, 12 K, 20 K, and 30 K) were prepared to investigate their cellular uptake by murine-derived macrophages. In a medium with FBS, the cellular uptake of FNP-PEGs was decreased as compared to a medium without FBS, indicating that protein corona on FNP-PEGs reduced cellular uptake. Bovine serum albumin (BSA) and hemoglobin (Hb) were detected as the most abundant components on all FNP-PEGs. Pre-coating of FNP-PEGs with BSA and Hb reduced the macrophage uptake in a medium without FBS, suggesting that these components might strengthen the stealth function of PEGs. Furthermore, there was more reduction in uptake of BSA- and Hb-coated FNP-PEGs from a medium with FBS than without FBS. BSA and Hb could be the stealth enhancement protein of FNP-PEGs in vitro.