Phenotypic and clinical characterization of low density neutrophils in patients with advanced lung adenocarcinoma.
Phenotypic and clinical characterization of low density neutrophils in patients with advanced lung adenocarcinoma.
复制标题
晚期肺腺癌患者低密度中性粒细胞的表型和临床特征
DOI：
10.18632/oncotarget.18771
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发表时间：
2017-10-31
期刊：
影响因子：
--
通讯作者：
Liu L
中科院分区：
文献类型：
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作者：
Liu Y;Hu Y;Gu F;Liang J;Zeng Y;Hong X;Zhang K;Liu L
目的 据报道，中性粒细胞的免疫抑制亚群低密度中性粒细胞 (LDN) 与多种疾病密切相关。本研究旨在探讨 LDN 与晚期肺腺癌之间的关联以及潜在机制。结果LDNs表面CD分子的表达水平与高密度中性粒细胞（HDNs）不同，与之前的研究一致。肺腺癌患者的 LDN/HDN 比率（而不是外周血单核细胞 (PBMC) 中 LDN 的百分比）显着高于健康对照。还观察到，当患者接受抗癌治疗时，该比率会下降，而当疾病复发时，该比率会上升。表皮生长因子受体（EGFR）突变阳性的患者的比例明显更高。比率和百分比均与CD8+T细胞呈正相关。尽管在肺腺癌患者中检测到TGF-β显着升高，但TGF-β与LDNs之间的关系并不明显。材料与方法采用流式细胞术测定52名肺腺癌患者和13名健康对照者外周血LDNs和HDNs水平。还检测到淋巴细胞和细胞因子。结论 肺腺癌患者中发现两种不同表型的中性粒细胞。此外，我们发现这些患者存在较高的LDNs/HDNs比例，这与疾病预后、EGFR突变和免疫状态不良有关。
Purpose An immunosuppressive subgroup of neutrophils, low density neutrophils (LDNs) was reported to be closely related to several diseases. This study was designed to explore the association between LDNs and advanced lung adenocarcinoma, as well as potential mechanisms. Results The expression levels of surface CD molecules on LDNs were different from high density neutrophils (HDNs), consistent with previous studies. The ratio of LDNs/HDNs, rather than the percentage of LDNs in peripheral blood mononuclear cells (PBMCs), was significantly higher in lung adenocarcinoma patients than healthy controls. It was also observed that the ratio decreased when patients received anti-cancer treatments, and increased when disease relapsed. Patients harboring positive epidermal growth factor receptor (EGFR) mutation had significantly higher ratios. Both the ratio and the percentage showed positive correlation with CD8+ T cells. Although significantly increased TGF-β was detected in lung adenocarcinoma patients, relationship between TGF-β and LDNs was not obvious. Materials and Methods LDNs and HDNs levels of peripheral blood from 52 lung adenocarcinoma patients and 13 healthy controls were determined by flow cytometry. Lymphocytes and cytokines were also detected. Conclusions Two kinds of neutrophils with different phenotypes were identified in lung adenocarcinoma patients. Besides, we found the existence of high ratio of LDNs/HDNs in these patients, which is related to disease prognosis, EGFR mutation and bad immune status.