1,3-DINITROBENZENE - TOXIC EFFECTS INVIVO AND INVITRO
1,3-DINITROBENZENE - TOXIC EFFECTS INVIVO AND INVITRO
复制标题
DOI：
10.1080/15287398109530024
复制
发表时间：
1981-01-01
期刊：
影响因子：
--
通讯作者：
CHRISTIAN, RT
中科院分区：
文献类型：
--
作者：
CODY, TE;WITHERUP, S;CHRISTIAN, RT
1，3-二硝基苯（1，3-DNB）的1%玉米油混悬液经口给药，LD_（50）为83 mg/kg，可信限为56-124 mg/kg。该化合物对两种性别的毒性相同。毒性体征包括行走活动减少、共济失调、虚弱、呼吸困难、心跳加快、发绀、昏迷和呼吸衰竭。当1，3-DNB以50、100和200 mg/l的浓度添加到每日饮用水中8周时，接受最高浓度的6只雄性大鼠中有3只在第4周死亡，另一只在第5周死亡。在雌性动物中，1只在第6周死亡，另1只在第7周死亡。其他动物都活了下来。两种性别动物的生长速率均降低，在供水中加入200 mg/l 1，3-DNB时，动物体重减轻。红细胞压积和Hb值出现轻度、一致性降低。在所有浓度下，两种性别的大鼠均出现脾脏肿大;在200 mg/l 1，3-DNB剂量下，所有大鼠均出现纤维化伴含铁血黄素沉积。睾丸萎缩明显，但对卵巢无影响。肝枯否细胞内有棕黄色沉积。己巴比妥诱导的睡眠时间减少。1，3-DNB以3、8或20 mg/l的剂量加入饮水中，连续16周，未见急性毒性反应。暴露8周后，接受20 mg/l剂量的雌性动物的生长逐渐迟缓;其他组的生长正常。20 mg/l剂量组5周和10周后，雄性动物的红细胞压积和Hb值出现中度降低。16周后，相同动物的1，3-DNB值正常。脾脏重量增加，并观察到含铁血黄素沉积。在20毫克/升剂量水平下，雄性睾丸重量减轻。在饮用水中暴露于3和8 mg/l 1，3-DNB 90天的雄性大鼠中观察到行为异常，表现为转轮活动增加。其他雄性大鼠也受到类似的暴露，但保持在活动平台上，显示活动增加，但增加不显著。在3-8 mg 1，3-DNB/l培养基中观察到对体外细胞的最小影响。在20-25毫克/升时，观察到生长减少50%。当暴露的1，3-DNB浓度超过56 mg/l时，所有细胞均被杀死。
When 1,3-dinitrobenzene (1,3-DNB) was given to rats orally as a 1% suspension in corn oil, the LD50 was 83 mg/kg with fiducial limits 56-124 mg/kg. The compound was equally toxic in both sexes. Signs of toxicity included reduction in ambulatory motion, ataxia, weakness, dyspnea, rapid heartbeat, cyanosis, coma and respiratory failure. When 1,3-DNB was added to the daily drinking water in concentrations of 50, 100 and 200 mg/l for 8 wk, 3 of 6 male rats receiving the highest concentration died during wk 4 and another died during wk 5. In females, 1 died during wk 6 and another during the wk 7. All other animals survived. Growth rate was reduced in both sexes and at 200 mg/l 1,3-DNB in the water supply the animals lost weight. There were mild, consistent reductions in hematocrit and Hb values. Enlarged spleens were present in both sexes at all concentrations; fibrosis with deposition of hemosiderin was present in all rats at 200 mg/l 1,3-DNB. Testicular atrophy was evident but there was no effect on the ovaries. A brown-yellow pigment was deposited in the Kupffer cells of the liver. Hexobarbital-induced sleep time decreased. When 3, 8 or 20 mg/l 1,3-DNB was provided daily in drinking water for 16 wk, no signs of acute toxicity were seen. Growth of females receiving 20 mg/l was retarded progressively after 8 wk of exposure; growth was normal in the other groups. Moderate reductions in hematocrit and Hb values occurred in males after 5 and 10 wk of 20 mg/l. 1,3-DNB values were normal in the same animals after 16 wk. Spleens were increased in weight and hemosiderin deposits were observed. Testes were reduced in weight among males at the 20 mg/l level. Behavioral abnormalities, in the form of increased activity in running wheels, were noted in male rats exposed to 3 and 8 mg/l 1,3-DNB in drinking water for 90 days. Other male rats similarly exposed, but kept on activity platforms showed increased activity but the increases were not significant. Minimal effects on cells in vitro were noted at 3-8 mg 1,3-DNB/l medium. Growth reduction by 50% was observed at 20-25 mg/l. All cells were killed when the concentration of 1,3-DNB to which they were exposed exceeded 56 mg/l.