Expression of E-cadherin in primary and metastatic prostate cancer.
Expression of E-cadherin in primary and metastatic prostate cancer.
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发表时间：
1996-05
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通讯作者：
L. Cheng;M. Nagabhushan;T. P. Pretlow;S. Amini;T. Pretlow
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作者：
L. Cheng;M. Nagabhushan;T. P. Pretlow;S. Amini;T. Pretlow
免疫组化研究表明，E-钙粘蛋白可能是一个有用的前列腺癌的预后标志物。以前的研究依赖于粗略选择的组织的低温恒温切片。许多前列腺癌，即使是广泛的，也是肉眼不可见的;许多其他的前列腺癌不能明显区分。全前列腺切除术后预后标志物的广泛适用性将取决于可应用于基于整个前列腺的组织病理学检查选择的组织的方法。我们的目的是研究E-cadherin在石蜡包埋的全前列腺和转移性前列腺癌中表达的可能性。柠檬酸盐缓冲液中的微波是用于证明石蜡包埋前列腺中的E-钙粘蛋白的五种方法中最好的，并用于调查来自44名患者的53例原发性前列腺癌和来自14名患者的淋巴结转移。前列腺癌转移到淋巴结表达较少（P = 0.008）E-cadherin比原发性前列腺癌。E-cadherin的表达与前列腺癌的组织学分化程度（Gleason分级）相关（P = 0.03，Ptrend = 0.003）。使用单克隆抗人E-钙粘蛋白（HECD-1）与微波在柠檬酸盐缓冲液中，然后通过免疫过氧化物酶染色与重金属增强的石蜡包埋组织中的E-钙粘蛋白的演示，将首次允许使用档案组织的前列腺癌和其他组织中的E-钙粘蛋白的预后研究。我们的研究结果是一致的假设，积极的前列腺癌表现出减少表达的E-钙粘蛋白，并证明了长期预后研究的可行性，这种分子通常是多个前列腺癌中发现的整体，福尔马林固定，石蜡包埋切除的前列腺。
Immunohistochemical studies have suggested that E-cadherin may be a useful prognostic marker in prostate cancer. Previous studies have depended on cryostat sections of tissues selected grossly. Many prostate cancers, even when extensive, are not visible grossly; many others cannot be demarcated sharply grossly. The wide applicability of prognostic markers after total prostatectomy will depend upon methods that can be applied to tissue selected based upon the histopathological examination of the entire prostate. Our purpose was to investigate the possibility that E-cadherin could be demonstrated in paraffin-embedded whole prostates and metastatic prostate cancer. Microwaving in citrate buffer was the best of five methods tested for the demonstration of E-cadherin in paraffin-embedded prostate and was used to investigate 53 primary prostate cancers from 44 patients and lymph node metastases from 14 patients. Metastases of prostate cancer to lymph nodes expressed less (P = 0.008) E-cadherin than primary prostate cancers. The expression of E-cadherin correlated with the histopathological differentiation (Gleason grade) of primary prostate cancers (P = 0.03, Ptrend = 0.003). The use of monoclonal anti-human E-cadherin (HECD-1) with microwaving in citrate buffer followed by immunoperoxidase staining with heavy metal enhancement for the demonstration of E-cadherin in paraffin-embedded tissue will, for the first time, allow the use of archival tissue for prognostic studies of E-cadherin in prostate cancer and other tissue. Our results are consistent with the hypothesis that aggressive prostate cancers exhibit decreased expression of E-cadherin and demonstrate the feasibility of long-term prognostic studies of this molecule in the usually multiple prostate cancers found in whole, formalin-fixed, paraffin-embedded resected prostates.