Diel variation in CC chemokine gene expression in the Japanese pufferfish Takifugu rubripes.
Diel variation in CC chemokine gene expression in the Japanese pufferfish Takifugu rubripes.
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日本河豚 Takifugu rubripes 中 CC 趋化因子基因表达的 Diel 变异。
DOI：
10.1007/s10126-020-09988-x
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发表时间：
2020
影响因子：
3
通讯作者：
Kono T.
中科院分区：
文献类型：
--
作者：
Tsutsui T;Onoue T;Hikima J;Sakai M;Kono T.
CC趋化因子是调节白细胞向损伤、感染或炎症部位转运的关键分子。近年来，一些哺乳动物趋化因子表现出受时钟基因调控的节律性表达。然而，硬骨鱼中趋化因子的节律性表达仍不清楚。本研究以模式鱼河豚为材料，研究了硬骨鱼CC趋化因子基因的昼夜变异。Diel变异分析表明，Clock(BMal1、Clock1、Per2、Rorα、Andrev-erbβ)和CC趋化因子(Ccl181、Ccl19和Ccl25l)基因在12：12光暗周期(LD12：12)条件下表现出双表达。CC趋化因子基因在转录调控区含有RORE(ccl18l、ccl19、ccl25l)和/或E-box(Ccl25l)基序。此外，在LD12：12条件下，不同时间(ZT)的脂多糖(LPS)刺激头肾可影响ccl181、ccl19和ccl251的表达，并分别观察到ZT12和ZT0(Ccl25l)和ZT16和ZT4(Ccl18landccl19)对内毒素刺激的高和低反应性。这些结果表明，某些鱼类CC趋化因子的表达受Clock蛋白调控的分子水平变化的影响，对细菌感染的反应性取决于时区。
CC chemokines are key molecules in the regulation of leukocyte trafficking to the site of injury, infection, or inflammation. In recent years, some mammalian chemokines have been shown to exhibit rhythmic expression, regulated by clock genes. However, the rhythmic expression of chemokines in teleost fish remains unknown. In the present study, the diel variation of teleost CC chemokine genes was investigated using the model fish,Fugu(Takifugu rubripes). Diel variation analysis revealed that clock (bmal1,clock1,per2,rorα, andrev-erbβ) and CC chemokine (ccl18l,ccl19, andccl25l) genes show diel expression under 12:12 light-dark cycle (LD12:12) conditions. CC chemokine genes, which exhibit diel expression, contain RORE (ccl18l,ccl19,ccl25l) and/or E-box (ccl25l) motifs in their transcription regulatory region. Moreover, in vitro head kidney stimulation with lipopolysaccharide (LPS) at different zeitgeber times (ZT) under LD12:12 conditions affected the degree ofccl18l,ccl19, andccl25lexpression; high and low responsiveness to LPS stimulation at ZT12 and ZT0 (ccl25l), and ZT16 and ZT4 (ccl18landccl19), respectively, were observed. These results suggest that the expression of some fish CC chemokines is affected by the diel variation regulated by clock proteins, and that responsiveness against bacterial infection depends on the time zone.