THE USE OF FRESHLY ISOLATED GILL EPITHELIAL-CELLS IN TOXICITY TESTING
THE USE OF FRESHLY ISOLATED GILL EPITHELIAL-CELLS IN TOXICITY TESTING
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DOI：
10.1016/0887-2333(95)00010-6
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发表时间：
1995-06-01
影响因子：
3.2
通讯作者：
ISOMAA, B
中科院分区：
文献类型：
--
作者：
LILIUS, H;SANDBACKA, M;ISOMAA, B
我们环境中的大量化学品，其中许多没有经过充分的毒性测试，证明有必要进行快速和可靠的体外测试来预测毒性。在水生毒理学中，细胞毒性测试的使用一直受到限制。在本研究中，通过使用荧光细胞活性探针Calcein-AM检测30种参比化学物质对细胞的毒性，评估了新鲜分离的虹鱼鳃上皮细胞在毒性测试中的有效性。将所得结果与已获得的化学物质在鲜鱼肝细胞中的EC(50)值、固定化大型水蚤的EC(50)值以及鱼类的LC(50)值进行了比较。对鳃上皮细胞和肝细胞的数据进行回归分析，相关系数为0.77(r(2)=0.59)。鳃上皮细胞对化学物质的敏感性略高于肝细胞。回归直线的斜率为0.79。这两种细胞对化学物质的敏感性均低于马尾藻和FISH，但鳃上皮细胞的EC(50)值与固定化马氏藻的EC(50)值以及FISH的LC(50)值比肝细胞的EC(50)值具有更好的一致性。
The large number of chemicals in our environment, many of which have been insufficiently tested for toxicity, justifies the need for rapid and reliable in vitro testing to predict toxicity. In aquatic toxicology the use of cellular toxicity tests has been limited. In the present study, the usefulness of freshly isolated gill epithelial cells from rainbow trout in toxicity testing was evaluated by testing the cytotoxicity of 30 reference chemicals to the cells using the fluorescent cell viability probe Calcein-AM. The results were compared with previously obtained EC(50) values for the chemicals in freshly isolated rainbow trout hepatocytes, EC(50) values for immobilization of Daphnia magna and literature data on LC(50) values for fish. Regression analysis of the data obtained with gill epithelial cells and hepatocytes showed a correlation coefficient of 0.77 (r(2) = 0.59). The gill epithelial cells were slightly more sensitive than hepatocytes to the chemicals. The slope of the regression line was 0.79. Both cell types were less sensitive than D. magna and fish to the chemicals, but the EC(50) values for gill epithelial cells showed a better concordance with EC(50) values for immobilization in D. magna and with literature data for fish LC(50) values than the hepatocyte EC(50) values.