Responses of cattle to two dosages of Brucella abortus strain RB51:: serology, clearance and efficacy
Responses of cattle to two dosages of Brucella abortus strain RB51:: serology, clearance and efficacy
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DOI：
10.1053/rvsc.1998.0251
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发表时间：
1999-04-01
影响因子：
2.4
通讯作者：
Cheville, NF
中科院分区：
文献类型：
--
作者：
Olsen, SC;Bricker, B;Cheville, NF
一种新的布鲁氏菌病疫苗，布鲁氏菌流产株RB51(SRB51)，目前在美国被推荐作为幼年疫苗使用，剂量在1×10(10)到3.4×10(10)集落形成单位(CFU)之间。这项研究的目的是比较SRB51最小和最大推荐剂量的反应。18头小母牛分别皮下接种1.6×10(10)CFU的SRB51、3.2×10(10)CFU的SRB51或生理盐水(n=6)。在接种1.6×10(10)CFU SRB51的6只动物中，有2只在接种14周后从颈部浅层淋巴结中恢复了疫苗毒株，但在任何接种3.2×10(10)CFU SRB51的牛身上没有发现疫苗毒株。SRB51剂量越大，抗体效价越高。流产杆菌菌株2308(S2308)攻击后对流产或感染的保护作用在两种SRB51剂量下相似，但高于非疫苗接种者的耐药性。疫苗毒株是在预计分娩前一周尸检时从一头小母牛和她的胎儿身上发现的。这项研究的数据表明，在推荐的剂量范围内，SRB51可以诱导类似的保护性免疫。SRB51疫苗可能会在一些牛身上持续到成年，但这种持续的发生率和意义尚不清楚。
A new brucellosis vaccine, Brucella abortus strain RB51 (SRB51), is currently recommended for use as a calfhood vaccine in the us at dosages between 1 x 10(10) and 3.4 x 10(10) colony-forming units (CFU). The purpose of the study reported here was to compare responses to minimal and maximal recommended SRB51 dosages. Eighteen heifer calves were vaccinated subcutaneously with 1.6 x 10(10) CFU of SRB51, 3.2 x 10(10) CFU of SRB51, or saline (n = 6 per treatment). The vaccine strain was recovered from the superficial cervical lymph node 14 weeks after vaccination in two of six animals that received 1.6 x 10(10) CFU SRB51, but not from any cattle vaccinated with 3.2 x 10(10) CFU SRB51. The higher SRB51 dosage stimulated greater antibody titres. Protection against abortion or infection following B. abortus strain 2308 (S2308) challenge was similar for both SRB51 dosages and greater than resistance of non-vaccinates. The vaccine strain was recovered from one heifer and her fetus at necropsy 1 week prior to estimated parturition. Data from this study suggests that SRB51 induces similar protective immunity across the recommended dosage range. The SRB51 vaccine may persist in some cattle into adulthood but the incidence and significance of this persistence remains unknown.