IDENTIFICATION OF AN IMMUNODOMINANT CYTOTOXIC LYMPHOCYTE-T RECOGNITION SITE IN GLYCOPROTEIN-B OF HERPES-SIMPLEX VIRUS BY USING RECOMBINANT ADENOVIRUS VECTORS AND SYNTHETIC PEPTIDES
IDENTIFICATION OF AN IMMUNODOMINANT CYTOTOXIC LYMPHOCYTE-T RECOGNITION SITE IN GLYCOPROTEIN-B OF HERPES-SIMPLEX VIRUS BY USING RECOMBINANT ADENOVIRUS VECTORS AND SYNTHETIC PEPTIDES
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DOI：
10.1128/jvi.65.3.1177-1186.1991
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发表时间：
1991-03-01
影响因子：
5.4
通讯作者：
JOHNSON, DC
中科院分区：
文献类型：
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作者：
HANKE, T;GRAHAM, FL;JOHNSON, DC
针对单纯疱疹病毒（HSV）多肽的细胞毒性T淋巴细胞（CTL）应答在从感染中恢复和预防潜伏期中起重要作用。我们先前已经表明糖蛋白B（gB）是C57 BL/6（H-2 B）和BALB/c（H-2d）小鼠中HSV特异性CTL识别的主要靶点，但在CBA/J（H-2k）小鼠中不识别（L. A. Witmer，K. L. Rosenthal，F. L.格雷厄姆，H。M. Friedman，A. Yee和D. C.约翰逊，J. Gen.病毒学。71：387-396，1990）。在这份报告中，我们利用腺病毒载体表达gB与各种删除本地化gB的免疫显性位点，reignized由H-2b限制性抗HSV CTL，残基462和594之间的区域。合成跨越该区域的重叠肽，并用于进一步将免疫显性位点定位于残基489至515，这是HSV 1型（HSV-1）和HSV-2毒株中高度保守的区域。11个氨基酸的肽显然只与K（B）主要组织相容性复合体基因产物相关，而与D（B）基因产物无关。相比之下，H-2d限制性CTL识别残基233和379之间的免疫显性位点。
Cytotoxic T-lymphocyte (CTL) responses to herpes simplex virus (HSV) polypeptides play an important role in recovery from infection and in preventing latency. We have previously shown that glycoprotein B (gB) is a major target recognized by HSV-specific CTLs in C57BL/6 (H-2b) and BALB/c (H-2d) mice but not in CBA/J (H-2k) mice (L. A. Witmer, K. L. Rosenthal, F. L. Graham, H. M. Friedman, A. Yee, and D. C. Johnson, J. Gen. Virol. 71:387-396, 1990). In this report, we utilize adenovirus vectors expressing gB with various deletions to localize an immunodominant site in gB, recongnized by H-2b-restricted anti-HSV CTLs, to a region between residues 462 and 594. Overlapping peptides spanning this region were synthesized and used to further localize the immunodominant site to residues 489 to 515, a region highly conserved in HSV type 1 (HSV-1) and HSV-2 strains. The 11-amino-acid peptide was apparently associated exclusively with the K(b) major histocompatibility complex gene product and not the D(b) gene product. In contrast, H-2d-restricted CTLs recongnized an immunodominant site between residues 233 and 379.