Enamel matrix derivative protein enhances production of matrixmetalloproteinase-2 by osteoblasts.
Enamel matrix derivative protein enhances production of matrixmetalloproteinase-2 by osteoblasts.
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DOI：
10.1186/1472-6831-14-85
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发表时间：
2014-07-10
期刊：
影响因子：
2.9
通讯作者：
Ikeo T
中科院分区：
文献类型：
--
作者：
Goda S;Inoue H;Takeuchi O;Ujii Y;Domae E;Ikeo T
基质金属蛋白酶（MMP）降解细胞外基质（ECM）并调节骨的重塑和再生。牙釉质基质衍生物（EMD）蛋白已在临床上用于牙周再生，但其分子机制尚不清楚。我们评估了基质金属蛋白酶 (MMP) 在调节成胚细胞样细胞系 MG63 上 EMD 依赖性明胶降解中的作用。在存在或不存在 MMP-2 组织抑制剂的情况下，将 MG-63 细胞（成骨细胞系）与 100 μg/ml EMD 蛋白一起孵育 20 小时，然后在 DQ 明胶包被的板上孵育 4 小时。 MG-63细胞（1××106）与SB203580在37℃下预孵育30分钟，然后置于100μg/ml EMD蛋白中24小时。收集条件培养基并通过蛋白质印迹分析进行检测。 EMD 蛋白增强细胞介导的明胶降解，而这种降解可被 MMP 抑制剂 TIMP-2 抑制。此外，MG63 细胞以 P38 MAPK 依赖性方式响应 EMD 蛋白而产生 MMP-2。此外，SB203580 阻断 p38 MAPK 激活可显着抑制 MMP-2 活性形式的产生。 P38 MAPK 通路促进 EMD 激活的成骨细胞中 MMP-2 的表达，进而通过降解牙周结缔组织中的基质蛋白来刺激牙周再生。
Matrix metalloproteinases (MMPs) degrade the extracellular matrix (ECM) and regulate remodeling and regeneration of bone. Enamel matrix derivative (EMD) protein has been used clinically for periodontal regeneration, although its molecular mechanisms are not clear. We evaluated the role of matrix metalloproteinases (MMPs) in regulating EMD-dependent degradation of gelatin on oeoblast-like cell line MG63. MG-63 cells (osteoblast cell line) were incubated with 100 μg/ml EMD protein in the presence or absence of MMP-2 tissue inhibitor for 20 h followed by incubation on DQ-gelatin-coated plates for 4 h. MG-63 cells (1 × 106) were preincubated with SB203580 for 30 min at 37°C and were then placed in 100 μg/ml EMD protein for 24 h. Conditioned media were collected and detected by Western blot analysis. EMD protein enhanced cell-mediated degradation of gelatin, which was inhibited by the MMP inhibitor TIMP-2. Furthermore, MMP-2 was produced by MG63 cells in response to EMD protein in a P38 MAPK-dependent manner. In addition, blocking of p38 MAPK activation by SB203580 significantly inhibited generation of the active form of MMP-2. P38 MAPK pathway promotes expression MMP-2 in EMD activated osteoblasts, which in turn stimulates periodontal regeneration by degrading matrix proteins in periodontal connective tissue.
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影响因子：
4.3
作者：
Goda, Seiji;Kaneshita, Yuki;Domae, Naochika
通讯作者：
Domae, Naochika
影响因子：
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作者：
Goda, S.;Inoue, H.;Domae, N.
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影响因子：
3.6
作者：
Kemoun, Philippe;Laurencin-Dalicieux, Sara;Salles, Jean-Pierre
通讯作者：
Salles, Jean-Pierre
影响因子：
4.3
作者：
Cochran, DL;Jones, A;King, GN
通讯作者：
King, GN