Molecular analysis of defect healing in rat diaphyseal bone
Molecular analysis of defect healing in rat diaphyseal bone
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DOI：
10.1292/jvms.63.603
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发表时间：
2001-06-01
影响因子：
1.2
通讯作者：
Neer, RM
中科院分区：
文献类型：
--
作者：
Chiba, S;Okada, K;Neer, RM
通过原位杂交分析成年雄性大鼠钻孔缺损愈合过程中成骨细胞标志物信使核糖核酸（mRNA）的空间表达。术后3天缺损处充满血肿，表达I型胶原mRNA。第 7 天，血肿被纤维组织取代，然后在第 10 天被来自髓内间隙的新骨小梁取代。分别。 I 型胶原、甲状旁腺激素 1 受体 (PTH1R) 和碱性磷酸酶 (ALP) 的 mRNA 在新形成的骨小梁附近的纤维组织的相同细胞群以及新形成的骨小梁内衬的成骨细胞中表达。带有破骨细胞的造血骨髓随后侵入该区域。也来自髓内空间，到第 21 天替换所有新骨小梁，除了薄的骨膜下层。 I型胶原蛋白、PTH1R和ALP的mRNA在骨膜表面薄层上表达。尽管软骨形成在组织学上不可见，但在新形成的骨小梁内衬的大多数成骨细胞中微弱地检测到 II 型胶原蛋白的 mRNA。
Spatial expression of messenger ribonucleic acid (mRNA) for osteoblastic marker in drill hole defect healing of adult male rats was analyzed by in situ hybridization. The defect was filled with hematoma 3 days after surgery, expressing Type I collagen mRNA. Hematoma was replaced with fibrous tissue on day 7, and then with new trabecular bone on day 10, originated from the intra-medullary space. respectively. mRNA for Type I collagen, parathyroid hormone 1 receptor (PTH1R), and alkaline phosphatase (ALP) were expressed in the same cell population of fibrous tissue adjacent to newly-formed trabecular bone, and in osteoblasts lining the newly-formed trabecular bone. Hematopoietic marrow with osteoclasts subsequently invaded the region. also from the intra-medullary space, replacing all the new trabecular bone by day 21, except for a thin sub-periosteal layer. mRNA for Type I collagen, PTH1R and ALP was expressed on the periosteal surface of thin layer. Although cartilage formation was not histologically visible, mRNA for Type II collagen was weakly detected in the majority of osteoblasts lining the newly-formed trabecular bone.