Effect of prostaglandin E1-induced elevation of cyclic AMP on glucose repression in the lactic streptococci.
Effect of prostaglandin E1-induced elevation of cyclic AMP on glucose repression in the lactic streptococci.
复制标题
前列腺素 E1 诱导的环 AMP 升高对乳酸链球菌葡萄糖抑制的影响。
DOI：
10.1139/m80-009
复制
发表时间：
1980
影响因子：
2.8
通讯作者：
D. E. Talburt
中科院分区：
文献类型：
--
作者：
T. Ratliff;R. Stinson;D. E. Talburt
在乳酸链球菌C2、乳脂链球菌C10、二乙酰乳酸链球菌18-16和嗜热链球菌C3中观察到环腺苷3 '，5' -单磷酸（cAMP）活性。从S.乳酸菌C2的结果表明，cAMP相关酶腺苷酸环化酶和磷酸二酯酶也存在。体外实验表明，前列腺素E1（PGE）刺激腺苷酸环化酶使cAMP浓度增加约5倍，体内研究表明，PGE处理S。lactis C2使细胞内cAMP浓度增加两倍。前列腺素E诱导的细胞内cAMP水平升高可防止葡萄糖对β-D-磷酸半乳糖苷半乳糖水解酶合成的抑制。
Cyclic adenosine 3', 5' -monophosphate (cAMP) activity was observed in Streptococcus lactis C2, Streptococcus cremoris C10, Streptococcus diacetlactis 18-16, and Streptococcus thermophilus C3. In vitro assays of cell-free extracts obtaianed from S. lactis C2 showed that the cAMP-associated enzymes adenyl cyclase and phosphodiesterase were also present. In vitro experiments showed that prostaglandin E1 (PGE) stimulation of adenyl cyclase increased cAMP concentrations approximately fivefold, and in vivo studies showed that PGE treatment of S. lactis C2 increased intracellular cAMP concentrations twofold. Futhermore, PGE-induced elevation of intracellular cAMP levels was shown to prevent the repression of ss-D-phosphogalactoside galactohydrolase synthesis by glucose.