Protein Microarray On-Demand: A Novel Protein Microarray System
Protein Microarray On-Demand: A Novel Protein Microarray System
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DOI：
10.1371/journal.pone.0003265
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发表时间：
2008-09-24
期刊：
影响因子：
3.7
通讯作者：
Munroe, David J.
中科院分区：
文献类型：
--
作者：
Chatterjee, Deb K.;Sitaraman, Kalavathy;Munroe, David J.
We describe a novel, simple and low-cost protein microarray strategy wherein the microarrays are generated by printing expression ready plasmid DNAs onto slides that can be converted into protein arrays on-demand. The printed expression plasmids serve dual purposes as they not only direct the synthesis of the protein of interest; they also serve to capture the newly synthesized proteins through a high affinity DNA-protein interaction. To accomplish this we have exploited the high-affinity binding (similar to 3-7 x 10(-13) M) of E. coli Tus protein to Ter, a 20 bp DNA sequence involved in the regulation of E. coli DNA replication. In our system, each protein of interest is synthesized as a Tus fusion protein and each expression construct directing the protein synthesis contains embedded Ter DNA sequence. The embedded Ter sequence functions as a capture reagent for the newly synthesized Tus fusion protein. This "all DNA'' microarray can be converted to a protein microarray on-demand without need for any additional capture reagent..
