Aberrant p16 promoter methylation among Greek lung cancer patients and smokers:: correlation with smoking
Aberrant p16 promoter methylation among Greek lung cancer patients and smokers:: correlation with smoking
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DOI：
10.1097/01.cej.0000236260.26265.d6
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发表时间：
2007-10-01
影响因子：
2.4
通讯作者：
Kouidou, Sofia
中科院分区：
文献类型：
--
作者：
Georgiou, Elisavet;Valeri, Rozalia;Kouidou, Sofia
遗传和环境因素（饮食和吸烟）影响肺癌流行病学，并诱导表观遗传修饰，应在个体人群中进行评估。我们使用两阶段甲基化特异性聚合酶链反应分析了希腊非小细胞肺癌患者和吸烟者的p16甲基化。包括150例癌组织和邻近非癌组织标本、患者支气管冲洗液和痰液，以及48例无病吸烟者标本（主要是痰液）。p16甲基化在患者的活检组织（82.85%）和支气管冲洗液（非小细胞肺癌，80.35%;小细胞肺癌，16.66%）中非常常见，而且在邻近的非癌组织（45.71%）中也是如此。p16甲基化与细胞学检查阳性的符合率为51.78%。在无症状细胞学阴性吸烟者（22.5%）和慢性阻塞性肺疾病患者（8例中的3例）的痰液中也观察到甲基化。在无病个体中，甲基化仅与重度吸烟相关（> 50包-年，P < 0.001），并且在男性和女性无病吸烟者中存在差异。总之，p16甲基化在非小细胞肺癌患者中非常常见，并且仅在无病吸烟者中与大量吸烟相关。
Genetic and environmental factors (dietary and smoking) influence lung cancer epidemiology and induce epigenetic modifications that should be assessed in individual populations. We analyzed p16 methylation among Greek non-small cell lung carcinoma patients and smokers using two-stage methylation-specific polymerase chain reaction. One hundred and fifty specimens from cancerous and adjacent non-cancerous tissue, bronchial washings and sputum from patients and 48 specimens, mostly sputum, from disease-free smokers were included. p16 methylation was very frequent in biopsies (82.85%) and bronchial washings (non-small cell lung carcinoma, 80.35%; small cell lung carcinoma, 16.66%) from patients, but also in adjacent non-cancerous tissue (45.71%). Concordance of p16 methylation and positivity by cytological examination was 51.78%. Methylation was also observed in sputum from asymptomatic cytology-negative smokers (22.5%) and chronic obstructive pulmonary disease patients (three of eight). Among disease-free individuals, methylation correlated only with heavy smoking (> 50 pack-years, P < 0.001) and differed among male and female disease-free smokers. In summary, p16 methylation is very frequent among non-small cell lung carcinoma patients, and correlates with heavy cigarette consumption only in disease-free smokers.