ZmHSP16.9, a cytosolic class I small heat shock protein in maize (Zea mays), confers heat tolerance in transgenic tobacco
ZmHSP16.9, a cytosolic class I small heat shock protein in maize (Zea mays), confers heat tolerance in transgenic tobacco
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ZmHSP16.9 是玉米 (Zea mays) 中的一种胞质 I 类小热休克蛋白，赋予转基因烟草耐热性
DOI：
10.1007/s00299-012-1262-8
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发表时间：
2012-08-01
影响因子：
6.2
通讯作者：
Yang, Xinghong
中科院分区：
文献类型：
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作者：
Sun, Liping;Liu, Yang;Yang, Xinghong
各种生物体在高温和其他胁迫下产生热休克蛋白。热休克蛋白，包括小热休克蛋白（sHSP）在抗逆性中的作用尚未得到充分的研究。为了提高我们对sHSPs的认识，我们从玉米中分离出zmhsp16.9。序列比对和系统发育分析表明，这是一个细胞质I类shsp . zmhsp16.9，在40°C处理下在根、叶和茎组织中表达，并在热胁迫和外源H2O2的作用下上调。zmhsp16.9基因在转基因烟草中的过表达，通过增加种子发芽率、根长和抗氧化酶活性，使烟草对高温和氧化胁迫具有抗性。这些结果支持了zmhsp16.9在植物热胁迫响应中的积极作用。zmhsp16.9的过表达增强了转基因烟草对高温和氧化胁迫的耐受性。
Various organisms produce HSPs in response to high temperature and other stresses. The function of heat shock proteins, including small heat shock protein (sHSP), in stress tolerance is not fully explored. To improve our understanding of sHSPs, we isolatedZmHSP16.9from maize. Sequence alignments and phylogenetic analysis reveal this to be a cytosolic class I sHSP.ZmHSP16.9expressed in root, leaf and stem tissues under 40 °C treatment, and was up-regulated by heat stress and exogenous H2O2. Overexpression ofZmHSP16.9in transgenic tobacco conferred tolerance to heat and oxidative stresses by increased seed germination rate, root length, and antioxidant enzyme activities compared with WT plants. These results support the positive role ofZmHSP16.9in response to heat stress in plant.Key messageThe overexpression ofZmHSP16.9enhanced tolerance to heat and oxidative stress in transgenic tobacco.