Two alternatively spliced mouse urokinase receptor mRNAs with different histological localization in the gastrointestinal tract.
Two alternatively spliced mouse urokinase receptor mRNAs with different histological localization in the gastrointestinal tract.
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DOI：
10.1083/jcb.115.6.1763
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发表时间：
1991-12
期刊：
影响因子：
--
通讯作者：
Danø K
中科院分区：
文献类型：
--
作者：
Kristensen P;Eriksen J;Blasi F;Danø K
分离了两个小鼠尿激酶型纤溶酶原激活物受体（muPAR）cDNA：muPAR 1与人尿激酶型纤溶酶原激活物受体同源，而muPAR 2编码与muPAR 1共享前133个残基的199个残基蛋白。小鼠基因组DNA测序表明，这两种不同的mRNA通过选择性剪接产生。原位杂交结果显示两种mRNA在小鼠胃粘膜中的表达存在差异。muPAR1 mRNA位于靠近固有层的尿激酶型纤溶酶原激活物产生结缔组织细胞的腔上皮细胞中，表明纤溶酶产生受不同细胞的合作控制，所述细胞可能在胃上皮细胞的释放中起作用。muPAR2 mRNA在基底上皮细胞中表达，并且推导的蛋白质序列包括受体配体结合结构域，但省略了参与糖脂介导的膜锚定的区域，这表明muPAR2可能编码分泌的uPA结合蛋白。
Two mouse urokinase-type plasminogen activator receptor (muPAR) cDNAs were isolated: muPAR1 is homologous to the human urokinase-type plasminogen activator receptor while muPAR2 codes for a 199 residue protein sharing the first 133 residues with muPAR1. Mouse genomic DNA sequencing indicates that the two different mRNAs arise by alternative splicing. In situ hybridization showed differential expression of the two mRNAs in mouse gastric mucosa. muPAR1 mRNA is located in luminal epithelial cells situated close to urokinase-type plasminogen activator- producing connective tissue cells of the lamina propria, pointing to plasmin generation controlled by the cooperation of different cells that may play a role in the release of gastric epithelial cells. muPAR2 mRNA is expressed in the basal epithelial cells, and the deduced protein sequence includes the receptor ligand binding domain, but omits the region involved in glycolipid-mediated membrane anchoring, suggesting that muPAR2 may code for a secreted uPA binding protein.