ANALYSIS OF PERITONEAL-MACROPHAGES IN CONTINUOUS AMBULATORY PERITONEAL-DIALYSIS PATIENTS
ANALYSIS OF PERITONEAL-MACROPHAGES IN CONTINUOUS AMBULATORY PERITONEAL-DIALYSIS PATIENTS
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DOI：
10.1038/ki.1984.209
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发表时间：
1984-01-01
影响因子：
19.6
通讯作者：
DOUGLAS, SD
中科院分区：
文献类型：
--
作者：
GOLDSTEIN, CS;BOMALASKI, JS;DOUGLAS, SD
研究人员将持续动态腹膜透析（CAPD）患者的腹膜巨噬细胞（PMC）与健康志愿者的腹膜巨噬细胞以及来自CAPD患者、血液透析患者和健康志愿者的外周血单核细胞（PBM）进行了比较。CAPD患者的PMC与健康志愿者的PMC和PBM形态相似。细胞表面存在HLA-DR抗原和Fc受体。与健康志愿者的PBM相比，这些单核细胞在长期培养中具有相似的顺序形态学变化。CAPD患者的PMC的吞噬能力、过氧化氢生成和细菌活性与健康志愿者的PBM相同。趋化性和类二十碳酸前体摄取研究表明，来自CAPD患者的PMC可能是相对不成熟的骨髓来源细胞。尽管这些细胞作为吞噬细胞功能良好，但需要进一步研究来确定它们的免疫能力，其中许多成分在分化为成熟巨噬细胞的过程中发育，因此可能在CAPD患者中缺乏。
Peritoneal macrophages (PMC) from patients undergoing continuous ambulatory peritoneal dialysis (CAPD) were compared to peritoneal macrophages from healthy volunteers and to peripheral blood monocytes (PBM) from CAPD patients, hemodialysis patients, and healthy volunteers. PMC from CAPD patients had morphology similar to PMC and PBM from healthy volunteers. HLA-DR antigen and Fc receptors were present on the cell surface. These monocytes had similar sequential morphologic changes in long-term culture compared to PBM from healthy volunteers. Phagocytosis, hydrogen peroxide generation and bacterial activity were the same in PMC from CAPD patients as in PBM from healthy volunteers. Chemotaxis and eicosanoid precursor uptake studies suggest that PMC from CAPD patients may be relatively immature bone-marrow-derived cells. Although these cells function well as phagocytes, further study is warranted to define their immune competence, many components of which develop during differentiation into mature macrophages and may therefore be deficient in patients undergoing CAPD.