Selective toxicity of ZnO nanoparticles toward Gram-positive bacteria and cancer cells by apoptosis through lipid peroxidation
Selective toxicity of ZnO nanoparticles toward Gram-positive bacteria and cancer cells by apoptosis through lipid peroxidation
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DOI：
10.1016/j.nano.2010.10.001
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发表时间：
2011-04-01
影响因子：
5.4
通讯作者：
Manivannan, Govindasamy
中科院分区：
文献类型：
--
作者：
Premanathan, Mariappan;Karthikeyan, Krishnamoorthy;Manivannan, Govindasamy
纳米颗粒越来越多地被认为是其在生物应用，包括纳米医学的效用。本研究探讨了氧化锌（ZnO）纳米颗粒对原核和真核细胞的毒性。采用人白血病细胞（HL 60）和正常人外周血单个核细胞（PBMCs）研究了ZnO对哺乳动物细胞的细胞毒性。氧化锌的抗菌活性也被测试对革兰氏阴性细菌大肠杆菌和铜绿假单胞菌，以及革兰氏阳性细菌金黄色葡萄球菌，和效果与革兰氏阳性比革兰氏阴性菌更明显。ZnO纳米颗粒对HL 60癌细胞的杀伤能力优于正常人PBMC。纳米颗粒增强了脂质体膜中超声诱导的脂质过氧化作用。这项工作提出了两种机制的潜在的ZnO的毒性：（i）参与的产生活性氧（ROS）和（ii）诱导细胞凋亡。这项工作还揭示了ZnO纳米颗粒在治疗癌症中的潜在用途，因为它们对癌细胞具有选择性毒性。来自临床编辑：氧化锌对细菌的毒性与活性氧的产生和诱导细胞凋亡有关。有趣的是，这些影响在人髓性白血病细胞（HL 60）比正常外周血单核细胞差异更大。(C)2011 Elsevier Inc. All rights reserved.
Nanoparticles are increasingly recognized for their utility in biological applications including nanomedicine. The present study investigated the toxicity of zinc oxide (ZnO) nanoparticles toward prokaryotic and eukaryotic cells. Cytotoxicity of ZnO to mammalian cells was studied using human myeloblastic leukemia cells (HL60) and normal peripheral blood mononuclear cells (PBMCs). Antibacterial activity of ZnO was also tested against the Gram-negative bacteria Escherichia coli and Pseudomonas aeruginosa, as well as the Gram-positive bacterium Staphylococcus aureus, and the effect was more pronounced with the Gram-positive than the Gram-negative bacteria. ZnO nanoparticles exhibited a preferential ability to kill cancerous HL60 cells as compared with normal PBMCs. The nanoparticles enhanced ultrasound-induced lipid peroxidation in the liposomal membrane. The work suggested two mechanisms underlying the toxicity of ZnO: (i) involvement of the generation of reactive oxygen species (ROS) and (ii) induction of apoptosis. The work also revealed potential utility of ZnO nanoparticles in the treatment of cancer, for their selective toxicity to cancer cells.From the Clinical Editor: The toxicity of zinc oxide to bacteria was related to the generation of reactive oxygen species and to the induction of apoptosis. Interestingly, these effects were differentially greater in human myeloblastic leukemia cells (HL60) than normal peripheral blood mononuclear cells. (C) 2011 Elsevier Inc. All rights reserved.