Elevated circulating microRNA-122 is associated with obesity and insulin resistance in young adults
Elevated circulating microRNA-122 is associated with obesity and insulin resistance in young adults
复制标题
循环 microRNA-122 升高与年轻人肥胖和胰岛素抵抗相关
DOI：
10.1530/eje-14-0867
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发表时间：
2015-03-01
影响因子：
5.8
通讯作者：
Wang, Weiqing
中科院分区：
文献类型：
--
作者：
Wang, Rui;Hong, Jie;Wang, Weiqing
目的：微小RNA（miRNA）参与肥胖的调节，但功能研究尚未得出结论。检查人类循环 miRNA 水平与肥胖和胰岛素敏感性的关联可能会带来更好的见解。 设计和方法：从 112 名肥胖和对照受试者（50.0% 男性）收集的血清样本被随机分为四个池（每个肥胖或对照池中有 28 个样本）。通过微阵列检测全基因组循环 miRNA 谱。在来自 123 名肥胖（46.7% 男性）和 107 名对照（50.0% 男性）年轻人的个体血清样本中选择并验证了升高的 miR-122。进一步评估了循环 miR-122 水平与肥胖、胰岛素抵抗、脂质谱和肝酶相关参数之间的关联。结果：发现与对照受试者相比，肥胖患者血清中发现 34 种 miRNA 表达不同（P
Objective: MicroRNAs (miRNAs) are involved in the regulation of adiposity, but functional studies have yielded inconclusive results. Examining the associations of circulating miRNAs levels with obesity and insulin sensitivity in humans may lead to improved insights.Design and methods: Serum samples collected from 112 obese and control subjects (50.0% men) were randomly divided and combined into four pools (28 samples in each obese or control pool). The genome-wide circulating miRNA profiles were detected via microarray. Elevated miR-122 was selected and validated in individual serum samples from 123 obese (46.7% men) and 107 control (50.0% men) young adults. Associations between circulating miR-122 levels and parameters related to adiposity, insulin resistance, lipid profiles and hepatic enzymes were further assessed.Results: Thirty-four miRNAs were found to be expressed differently in the sera of obese patients compared with control subjects (P