Preservation of the inner retina in retinitis pigmentosa - A morphometric analysis
Preservation of the inner retina in retinitis pigmentosa - A morphometric analysis
复制标题
DOI：
10.1001/archopht.1997.01100150513011
复制
发表时间：
1997-04-01
影响因子：
--
通讯作者：
Milam, AH
中科院分区：
文献类型：
--
作者：
Santos, A;Humayun, MS;Milam, AH
目的：目的：探讨视网膜色素变性（RP）患者视网膜内层的保存程度，方法：对21例RP患者和19例年龄匹配的正常人的黄斑切片进行分析。将患眼分为2组：重度和中度RP。细胞核计数的外核，内核，神经节细胞层内30个100 μ m的间隔，从小凹到1500 μ m eccentricity.Results：统计学显着（P小于或等于.05）的损失的外核和神经节细胞层是存在于中度和重度RP组相比，与对照组。然而，即使在严重RP组中，30%的神经节细胞组织学完整。同样，78%和88%的内核层细胞保留在重度和中度RP组，分别。不同的遗传模式显示没有统计学上的显着差异，在任何的视网膜layers.Conclusions：尽管有统计学意义（P小于或等于.05）的损失，发现在所有视网膜层的细胞，很大比例的视网膜内层神经元保持组织学完整。目前的实验治疗，如感光细胞移植和植入的视觉假体，是基于这样的前提下，一些内部的视网膜神经元被保存后死亡的光感受器在RP。我们的观察支持这一假设。
Objective: To determine the extent of preservation in the inner retina in retinitis pigmentosa (RP).Methods: We analyzed sectioned maculae of 21 post-mortem eyes with RP and 19 age-matched, normal, post-mortem eyes. Eyes were divided into 2 groups: severe and moderate RP. Cell nuclei were counted in the outer nuclear, inner nuclear, and ganglion cell layers within thirty 100-mu m intervals from the foveola to 1500-mu m eccentricity.Results: Statistically significant (P less than or equal to.05) loss of both the outer nuclear and ganglion cell layers was present in the groups with moderate and severe RP when compared with the control groups. However, even in the group with severe RP, 30% of the ganglion cells were histologically intact. Similarly, 78% and 88% of the inner nuclear layer cells were preserved in the groups with severe and moderate RP, respectively. Different inheritance modes showed no statistically significant differences in any of the retinal layers.Conclusions: Despite a statistically significant (P less than or equal to.05) loss of cells found in all retinal layers, a large percentage of the inner retinal neurons remained histologically intact. Current experimental therapies, such as photoreceptor transplantation and implantation of a visual prosthesis, are based on the premise that some inner retinal neurons are preserved after death of photoreceptors in RP. Our observations support this assumption.