Mast Cell Activation Test (MAT)
Mast Cell Activation Test (MAT)
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DOI：
10.1007/978-1-0716-0696-4_19
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发表时间：
2020-01-01
期刊：
影响因子：
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通讯作者：
Bulfone-Paus, Silvia
中科院分区：
文献类型：
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作者：
Bahri, Rajia;Bulfone-Paus, Silvia
肥大细胞（MC）活化试验是一个强大的体外工具，探索过敏MC反应。在这里，我们描述了肥大细胞活化试验（MAT）的使用，该试验利用从外周血祖细胞产生的人原代MC，用患者血清过夜致敏并用过敏原活化。流式细胞术用于评估活化标志物CD 63的表达变化，细胞脱颗粒百分比定义为CD 63（+）阳性MC的百分比。
The mast cell (MC) activation assay is a robust in vitro tool for exploring MC reactivity in allergy. Here we describe the use of the mast cell activation test (MAT) that makes use of human primary MCs generated from peripheral blood progenitors, sensitized overnight with patients' sera and activated with allergens. Flow cytometry is used to assess the changes in expression of the activation marker CD63, and the percentage of cell degranulation is defined as the percentage of CD63(+)-positive MCs.