Biochemical and Morphological Characterization of a Guanine Nucleotide Exchange Factor ARHGEF9 in Mouse Tissues.
Biochemical and Morphological Characterization of a Guanine Nucleotide Exchange Factor ARHGEF9 in Mouse Tissues.
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DOI：
10.1267/ahc.18009
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发表时间：
2018-06-26
影响因子：
2.4
通讯作者：
Nagata KI
中科院分区：
文献类型：
--
作者：
Ibaraki K;Mizuno M;Aoki H;Niwa A;Iwamoto I;Hara A;Tabata H;Ito H;Nagata KI
ARHGEF 9，也称为Collybistin，是Rho家族GTP酶的鸟嘌呤核苷酸交换因子，被认为在哺乳动物大脑中起重要作用。本研究制备了ARHGEF 9的特异性多克隆抗体anti-ARHGEF 9，并在小鼠组织尤其是脑组织中进行了表达分析。蛋白质印迹分析表明，ARHGEF 9在年轻成年小鼠中的组织依赖性表达谱，并强烈表明在大脑发育过程中的作用。免疫组织化学分析显示，ARHGEF 9在大脑皮层，海马和小脑的发育阶段依赖性表达谱。ARHGEF 9在培养的海马神经元中表现出部分定位于树突棘。从所得结果来看，抗ARHGEF 9被发现是ARHGEF 9生化和细胞生物学分析的有用工具。
ARHGEF9, also known as Collybistin, a guanine nucleotide exchange factor for Rho family GTPases, is thought to play an essential role in the mammalian brain. In this study, we prepared a specific polyclonal antibody against ARHGEF9, anti-ARHGEF9, and carried out expression analyses with mouse tissues especially brain. Western blotting analyses demonstrated tissue-dependent expression profiles of ARHGEF9 in the young adult mouse, and strongly suggested a role during brain development. Immunohistochemical analyses revealed developmental stage-dependent expression profiles of ARHGEF9 in cerebral cortex, hippocampus and cerebellum. ARHGEF9 exhibited partial localization at dendritic spines in cultured hippocampal neurons. From the obtained results, anti-ARHGEF9 was found to be a useful tool for biochemical and cell biological analyses of ARHGEF9.
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