Niche-Specific Impact of a Symbiotic Function on the Persistence of Microbial Symbionts within a Natural Host.
Niche-Specific Impact of a Symbiotic Function on the Persistence of Microbial Symbionts within a Natural Host.
复制标题
共生功能对自然宿主内微生物共生体持久性的特定影响。
DOI：
10.1128/aem.01770-16
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发表时间：
2016
影响因子：
4.4
通讯作者：
Miyashiro,Tim
中科院分区：
文献类型：
--
作者：
Verma,SubhashC;Miyashiro,Tim
微生物共生体的功能如何受到它们在宿主内的种群/联合体结构的影响仍然知之甚少。真鞭毛虫和费氏弧菌之间建立的共生关系是一种具有良好特征的宿主-微生物组合，其中费氏弧菌群体在宿主内的功能和结构是已知的：费氏弧菌群体从称为光器官的专用宿主结构中的不同隐窝空间产生生物发光。先前的研究表明，发光是费氏弧菌种群在发光器官内持续存在所必需的，而导致费氏弧菌发光的基因位点的缺失导致了持久性缺陷。在这项研究中，我们研究了生物发光对发光器内费氏弧菌种群结构的影响。我们报告说，持续性缺陷是隐窝I所特有的，隐窝I是新生光器官中发育最成熟的隐窝空间。这一结果为未来鱿鱼-弧菌共生的机制研究提供了有益的结构/功能关系的见解。此外，我们的报告强调了宿主发育计划对宿主-微生物相互作用的时空动力学的潜在影响。微生物的共生功能与其在宿主环境中的空间结构之间的关系仍然知之甚少。在这里，我们使用二元共生证明，宿主对微生物共生体的共生功能的要求仅限于特定的宿主环境。我们的结果还表明，微生物功能和宿主发育之间存在联系，这可能是更复杂的宿主-微生物相互作用的一个基本方面。
How the function of microbial symbionts is affected by their population/consortium structure within a host remains poorly understood. The symbiosis established between Euprymna scolopes and Vibrio fischeri is a well-characterized host-microbe association in which the function and structure of V. fischeri populations within the host are known: V. fischeri populations produce bioluminescence from distinct crypt spaces within a dedicated host structure called the light organ. Previous studies have revealed that luminescence is required for V. fischeri populations to persist within the light organ and that deletion of theluxgene locus, which is responsible for luminescence in V. fischeri, leads to a persistence defect. In this study, we investigated the impact of bioluminescence on V. fischeri population structure within the light organ. We report that the persistence defect is specific to crypt I, which is the most developmentally mature crypt space within the nascent light organ. This result provides insight into the structure/function relationship that will be useful for future mechanistic studies of squid-Vibrio symbiosis. In addition, our report highlights the potential impact of the host developmental program on the spatiotemporal dynamics of host-microbe interactions.IMPORTANCEMetazoan development and physiology depend on microbes. The relationship between the symbiotic function of microbes and their spatial structure within the host environment remains poorly understood. Here we demonstrate, using a binary symbiosis, that the host requirement for the symbiotic function of the microbial symbiont is restricted to a specific host environment. Our results also suggest a link between microbial function and host development that may be a fundamental aspect of the more complex host-microbe interactions.