Distribution of 5-Methyltetrahydrofolate and Folic Acid Levels in Maternal and Cord Blood Serum: Longitudinal Evaluation of Japanese Pregnant Women
Distribution of 5-Methyltetrahydrofolate and Folic Acid Levels in Maternal and Cord Blood Serum: Longitudinal Evaluation of Japanese Pregnant Women
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DOI：
10.3390/nu12061633
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发表时间：
2020-06-01
期刊：
影响因子：
5.9
通讯作者：
Yamashiro, Yuichiro
中科院分区：
文献类型：
--
作者：
Kubo, Yoshinori;Fukuoka, Hideoki;Yamashiro, Yuichiro
通常测量血液中的“总”叶酸盐以评估孕妇的叶酸盐状态。然而，叶酸是由许多代谢产物组成的。主要底物是5-甲基四氢叶酸（5-MTHF），叶酸（FA）是血液中非常小的未代谢物质。我们纵向评估5-MTHF，FA和总同型半胱氨酸的母亲和脐带血从日本孕妇。受试者为146名参加千叶妇幼健康研究（C-MACH）前瞻性队列研究的孕妇。在妊娠早期和晚期、分娩时以及从脐带血中获得血清。通过同位素稀释质谱法测量物质水平。5-MTHF和FA水平均低于强制性FA强化国家人群中孕妇报告的水平。随着胎龄的增加，血清5-MTHF水平下降，而血清FA水平仅在分娩时略低于妊娠早期。血清5-MTHF和总同型半胱氨酸之间的显着负相关，在所有检查时间，但与FA没有明显的关联。分娩时，脐带5-MTHF显著高于母体水平，而FA再次显示无显著相关性。这些结果表明，5-MTHF是积极运输到胎儿通过胎盘转运蛋白，并可能反映叶酸状态在怀孕期间作为一个重要的生理物种。
"Total" folate in blood has usually been measured to evaluate the folate status of pregnant women. However, folate is composed of many metabolites. The main substrate is 5-methyltetrahydrofolate (5-MTHF), with folic acid (FA) representing a very small component as an unmetabolized species in blood. We longitudinally evaluated 5-MTHF, FA and total homocysteine in maternal and cord blood from Japanese pregnant women. Subjects were 146 pregnant women who participated in the Chiba study of Mother and Child Health (C-MACH) prospective cohort study. Sera were obtained in early and late pregnancy, at delivery, and from cord blood. Species levels were measured by isotope-dilution mass spectrometry. Both 5-MTHF and FA levels were lower than reported levels from pregnant women in populations from countries with mandatory FA fortification. As gestational age progressed, serum 5-MTHF levels decreased, whereas serum FA levels were slightly reduced only at delivery compared to early pregnancy. A significant negative association between serum 5-MTHF and total homocysteine was shown at all examined times, but no associations with FA were evident. At delivery, cord 5-MTHF was significantly higher than maternal levels, while FA again showed no significant correlation. These results suggest that 5-MTHF is actively transported to the fetus through placental transporters and may reflect folate status during pregnancy as a physiologically important species.