Imaging relative stasis of the blood column in human retinal capillaries
Imaging relative stasis of the blood column in human retinal capillaries
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DOI：
10.1364/boe.10.006009
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发表时间：
2019-11-01
影响因子：
3.4
通讯作者：
Metha, Andrew
中科院分区：
文献类型：
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作者：
Bedggood, Phillip;Metha, Andrew
毛细血管流动主要由交替的红细胞和血浆组成，其速度随心动周期可预测地振荡。叠加在这种常规背景上的是可能破坏毛细血管交换的偶发事件：白色细胞通过、红细胞聚集、稀疏红细胞压积时期或异常缓慢的流动。这些事件不容易区分与测速或灌注映射。在这里，我们提出了一种方法来识别这些现象在视网膜毛细血管成像高帧率自适应光学，计算和表示pictorially通过时间在每个像素在短时期内的强度的自相关。上述现象表现为短暂出现的亮区，并在其他暗图像上传播。从正常受试者和I型糖尿病患者中获取数据，我们证明了该指标对健康和疾病中毛细血管内容物和流速变化的概念证明以及高灵敏度和特异性。建议的度量提供了一个有用的辅助测速和灌注映射在正常和异常的毛细血管血流的研究。(C)根据OSA开放获取出版协议的条款，2019年美国光学学会
Capillary flow largely consists of alternating red cells and plasma whose speed oscillates predictably with the cardiac cycle. Superimposed on this regular background are sporadic events potentially disruptive to capillary exchange: the passage of white cells, aggregates of red cells, epochs of sparse haematocrit, or unusually slow flow. Such events are not readily differentiated with velocimetry or perfusion mapping. Here we propose a method to identify these phenomena in retinal capillaries imaged with high frame-rate adaptive optics, by calculating and representing pictorially the autocorrelation of intensity through time at each pixel during short epochs. The phenomena described above manifest as bright regions which transiently appear and propagate across an otherwise dark image. Drawing data from normal subjects and those with Type I diabetes, we demonstrate proof of concept and high sensitivity and specificity of this metric to variations in capillary contents and rate of flow in health and disease. The proposed metric offers a useful adjunct to velocimetry and perfusion mapping in the study of normal and abnormal capillary blood flow. (C) 2019 Optical Society of America under the terms of the OSA Open Access Publishing Agreement