Chemical pyrophosphorylation of functionally diverse peptides.
Chemical pyrophosphorylation of functionally diverse peptides.
复制标题
功能多样的肽的化学焦磷酸化。
DOI：
10.1021/ja411737c
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发表时间：
2014
影响因子：
15
通讯作者：
Fiedler,Dorothea
中科院分区：
文献类型：
--
作者：
Marmelstein,AlanM;Yates,LisaM;Conway,JohnH;Fiedler,Dorothea
A highly selective and convenient method for the synthesis of pyrophosphopeptides in solution is reported. The remarkable compatibility with functional groups (alcohol, thiol, amine, carboxylic acid) in the peptide substrates suggests that the intrinsic nucleophilicity of the phosphoserine residue is much higher than previously appreciated. Because the methodology operates in polar solvents, including water, a broad range of pyrophosphopeptides can be accessed. We envision these peptides will find widespread applications in the development of mass spectrometry and antibody-based detection methods for pyrophosphoproteins.
