Neutralizing anti-F glycoprotein and anti-substance P antibody treatment effectively reduces infection and inflammation associated with respiratory syncytial virus infection
Neutralizing anti-F glycoprotein and anti-substance P antibody treatment effectively reduces infection and inflammation associated with respiratory syncytial virus infection
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DOI：
10.1128/jvi.76.14.6873-6881.2002
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发表时间：
2002-07-01
影响因子：
5.4
通讯作者：
Tripp, RA
中科院分区：
文献类型：
--
作者：
Haynes, LM;Tonkin, J;Tripp, RA
呼吸道合胞病毒（RSV）是介导婴幼儿下呼吸道疾病的最重要病毒。 RSV 感染与肺部炎症和 P 物质 (SP) 水平升高有关，使表达 SP 受体的气道和白细胞容易受到该肽的促炎作用的影响。本研究探讨了对 RSV 感染的 BALB/c 小鼠联合中和抗 F 糖蛋白和抗 SP 抗体治疗，以抑制 RSV 复制和与感染相关的炎症。 BALB/c 小鼠在 RSV 感染前用抗体进行预防性治疗，或在 RSV 感染后第 2 天或第 6 天进行治疗性治疗。使用抗SP抗体进行预防性或治疗性治疗可迅速减少肺部炎症细胞浸润并减少表达促炎细胞因子的细胞数量，而抗F抗体治疗可降低病毒滴度。结果表明，抗病毒和抗 SP 抗体联合治疗可能有效治疗 RSV 疾病。
Respiratory syncytial virus (RSV) is the most important virus mediating lower respiratory tract illness in infants and young children. RSV infection is associated with pulmonary inflammation and increased levels of substance P (SP), making the airways and leukocytes that express SP receptors susceptible to the proinflammatory effects of this peptide. This study examines combining neutralizing anti-F glycoprotein and anti-SP antibody treatment of RSV-infected BALB/c mice to inhibit RSV replication and inflammation associated with infection. BALB/c mice were prophylactically treated with antibody prior to RSV infection or were therapeutically treated at day 2 or 6 post-RSV infection. Prophylactic or therapeutic treatment with anti-SP antibodies promptly reduced pulmonary inflammatory cell infiltration and decreased the number of cells expressing proinflammatory cytokines, while anti-F antibody treatment reduced virus titers. The results suggest that combined anti-viral and anti-SP antibody treatment may be effective in treating RSV disease.