Normal learning ability of mice with a surgically exposed hippocampus
Normal learning ability of mice with a surgically exposed hippocampus
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手术暴露海马体的小鼠的正常学习能力
DOI：
10.1097/wnr.0b013e32835375b6
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发表时间：
2012
期刊：
影响因子：
1.7
通讯作者：
ら
中科院分区：
文献类型：
--
作者：
Sakaguchi;Ishikawa;ら
在大鼠和小鼠中，海马位于新皮质下方超过1 mm处。这种解剖学特征使得很难在实验上从体内大脑表面进入海马体。这个问题可以通过手术切除海马体上方的皮质组织来解决;然而，还没有研究这种“海马窗”手术是否保留了正常的海马功能。我们双侧抽吸成年雄性小鼠背海马上方的后顶叶皮质。与完整或假手术对照组相比，这些小鼠在水迷宫测试和情境恐惧条件反射中仍表现出正常的海马局部场电位、正常的运动活动和正常的认知能力。因此，暴露的海马制剂提供了一个有用的实验模型，研究海马的生理。
In rats and mice, the hippocampus lies beneath higher than 1 mm of the neocortex. This anatomical feature makes it difficult to experimentally access the hippocampus from the surface of the brain in vivo. This problem may be solved by surgical removal of the cortical tissue above the hippocampus; however, it has not been examined whether this ‘hippocampal window’surgery preserves the normal hippocampal function. We bilaterally aspirated the posterior parietal cortex above the dorsal hippocampus of adult male mice. These mice still exhibited normal local field potentials of the hippocampus, normal motor activity, and normal cognitive ability in the water-maze test and contextual fear conditioning, compared with intact or sham-operated controls. Thus, exposed hippocampal preparations provide a useful experimental model to study the physiology of the hippocampus.