Gerbil angiotensin II AT1 receptors are highly expressed in the hippocampus and cerebral cortex during postnatal development.
Gerbil angiotensin II AT1 receptors are highly expressed in the hippocampus and cerebral cortex during postnatal development.
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沙鼠血管紧张素 II AT1 受体在出生后发育过程中在海马和大脑皮层中高度表达。
DOI：
10.1016/s0306-4522(99)00514-x
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发表时间：
2000
期刊：
影响因子：
3.3
通讯作者：
Saavedra,JM
中科院分区：
文献类型：
--
作者：
Tonelli,L;Johren,O;Hoe,KL;Hauser,W;Saavedra,JM
越来越多的证据表明，血管紧张素II，经典已知的许多影响，调节盐和水的稳态，也参与大脑发育和认知功能，通过激活AT 1血管紧张素II受体。最近克隆的沙鼠AT 1受体在控制水-矿物质平衡的脑区表达，特别是在海马结构等边缘区高度表达。我们定量沙土鼠AT 1受体信使RNA的表达和受体结合的定量原位杂交和受体放射自显影，分别在海马结构和大脑皮层的沙土鼠在出生后的发展。受体信使RNA和结合从出生时就存在，并在海马CA 1和CA 2区和齿状回的出生后成熟过程中表现出逐渐和持续的增加。相反，在CA 3区，没有检测到结合，而受体信使RNA在出生后15天达到峰值，并在成人中消失。最高的受体信使RNA的表达和结合被发现在septomedial部分的CA 1区和间隔水平的CA 2区。我们检测到最高的受体信使RNA表达在出生后第一天在大脑半球的额外侧极。在这些领域，在额顶叶和岛叶皮层，受体信使RNA显着减少在出生后的生活。同样，我们发现受体信使RNA的表达在扣带，压后，嗅周和边缘下皮质较高的值在前两周的发展和表达下降的成人。然而，受体结合在大脑皮层，并没有减少在出生后的生活。在出生后成熟的沙鼠皮层和海马的受体信使RNA的表达和结合的差异的配置文件表明，在皮质海马系统的发展和功能的AT 1受体的作用。
Increasing evidence suggests that Angiotensin II, classically known from its many effects regulating salt and water homeostasis, is also involved in brain development and cognitive functions through activation of AT1Angiotensin II receptors. The recently cloned gerbil AT1receptor is expressed in brain areas controlling hydro-mineral homeostasis, and particularly highly expressed in limbic areas such as the hippocampal formation. We quantified the gerbil AT1receptor messenger RNA expression and receptor binding by quantitative in situ hybridization and receptor autoradiography, respectively, in the hippocampal formation and cerebral cortex of gerbils during postnatal development. The receptor messenger RNA and binding were present from birth and showed a gradual and sustained increase through postnatal maturation in the CA1 and CA2 regions of the hippocampus and in the dentate gyrus. Conversely, in the CA3 region, no binding was detected while receptor messenger RNA peaked at 15 days after birth and disappeared in the adult. The highest receptor messenger RNA expression and binding were found in the septomedial portions of the CA1 region and at septal levels of the CA2 region. We detected the highest receptor messenger RNA expression at postnatal day one in the frontolateral pole of the cerebral hemispheres. In these areas, and in the frontoparietal and insular cortex, receptor messenger RNA dramatically decreased during postnatal life. Similarly, we found receptor messenger RNA expression in the cingulate, retrosplenial, perirhinal and infralimbic cortex with higher values during the first two weeks of development and decreased expression in the adult. However, receptor binding in the cerebral cortex, did not decrease during postnatal life. The differential profile of receptor messenger RNA expression and binding in the gerbil cortex and hippocampus during postnatal maturation suggest a role for AT1receptors in the development and function of the corticohippocampal system.