Laser Sintered Porous Ti-6Al-4V Implants Stimulate Vertical Bone Growth
Laser Sintered Porous Ti-6Al-4V Implants Stimulate Vertical Bone Growth
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DOI：
10.1007/s10439-017-1831-7
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发表时间：
2017-08-01
影响因子：
3.8
通讯作者：
Schwartz, Zvi
中科院分区：
文献类型：
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作者：
Cheng, Alice;Cohen, David J.;Schwartz, Zvi
本研究的目的是检查3D种植体的能力与骨小梁启发的多孔性和微米/纳米粗糙表面，以提高垂直骨长入。通过激光烧结制备多孔Ti-6Al-4V结构，并对其进行处理以获得微/纳米粗糙表面。将男性和女性人成骨细胞接种在构建体上以分析细胞形态和反应。然后将种植体放置在大鼠颅骨上10周，以评估垂直骨长入、机械稳定性和骨整合。与固体激光烧结对照相比，所有成骨细胞在多孔结构上显示出更高水平的骨钙素、骨保护素、血管内皮生长因子和骨形态发生蛋白2。植入体内的多孔种植体导致平均3.1 +/- 0.6 mm（3）的垂直骨生长和种植体孔内的骨整合，并且具有显著高于固体种植体的拔出强度值。加入脱钙骨基质油灰并不能改善新骨形成和拔出强度。扫描电子图像和组织学结果证实了垂直骨生长。本研究表明，通过增材制造（具有基于骨小梁的多孔性）和构建后处理（具有微米/纳米表面粗糙度）制造的Ti-6Al-4V植入物可支持体内垂直骨生长，并表明这些植入物可在临床上用于增加具有挑战性的患者病例中的骨整合。
The objective of this study was to examine the ability of 3D implants with trabecular-bone-inspired porosity and micro-/nano-rough surfaces to enhance vertical bone ingrowth. Porous Ti-6Al-4V constructs were fabricated via laser-sintering and processed to obtain micro-/nano-rough surfaces. Male and female human osteoblasts were seeded on constructs to analyze cell morphology and response. Implants were then placed on rat calvaria for 10 weeks to assess vertical bone ingrowth, mechanical stability and osseointegration. All osteoblasts showed higher levels of osteocalcin, osteoprotegerin, vascular endothelial growth factor and bone morphogenetic protein 2 on porous constructs compared to solid laser-sintered controls. Porous implants placed in vivo resulted in an average of 3.1 +/- 0.6 mm(3) vertical bone growth and osseointegration within implant pores and had significantly higher pull-out strength values than solid implants. New bone formation and pull-out strength was not improved with the addition of demineralized bone matrix putty. Scanning electron images and histological results corroborated vertical bone growth. This study indicates that Ti-6Al-4V implants fabricated by additive manufacturing to have porosity based on trabecular bone and post-build processing to have micro-/nano-surface roughness can support vertical bone growth in vivo, and suggests that these implants may be used clinically to increase osseointegration in challenging patient cases.