Identification of CD244-expressing myeloid-derived suppressor cells in patients with active tuberculosis
Identification of CD244-expressing myeloid-derived suppressor cells in patients with active tuberculosis
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活动性结核病患者中表达 CD244 的骨髓源性抑制细胞的鉴定
DOI：
10.1016/j.imlet.2013.12.003
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发表时间：
2014-03-01
影响因子：
4.4
通讯作者：
Cheng, Xiaoxing
中科院分区：
文献类型：
--
作者：
Yang, Bingfen;Wang, Xinjing;Cheng, Xiaoxing
活动性结核病的发展伴随着免疫抑制，近年来已广泛探索了其潜在机制。MDSC是一组异质性的未成熟和祖骨髓细胞，对自然免疫和适应性免疫都具有很强的免疫抑制能力。在我们对活动性TB患者PBMC中表达CD 244（2B 4）的细胞的分析中，鉴定了CD 3（-）CD 244（高）亚群。流式细胞仪细胞群匹配显示几乎所有的CD 3（-）CD 244（high）细胞都是CD 3（-）HLA-DR（-）CD 11b（int）CD 33（+）细胞。CD 3-CD 244（高）细胞群具有CD 3（-）CD 19（-）CD 56（-）CD 15（-）CD 66 b（-）CD 33（+）CD 11b（+）CD 14（-）HLA-DRneg/low的表型，这与先前报道的人类MDSC一致。与健康对照组相比，活动性TB患者的CD 3（-）CD 244（高）细胞频率较高。CD 3（-）CD 244（高）细胞群体具有高水平的NOS 2表达，并且与CD 4（+）和CD 8（+）T细胞的活化和有效分子产生呈负相关。结论：CD 3（-）CD 244（high）细胞具有MDSCs的表型，CD 244可作为人CD 3（-）HLA（-）DR（-）CD 11b（int）CD 33（+）MDSCs的标志物。(C)2013爱思唯尔有限公司版权所有。
Development of active TB is accompanied by immune suppression and the underlining mechanisms have been explored extensively in recent years. MDSCs are a heterogeneous group of immature and progenitor myeloid cells with strong immunosuppressive ability for both natural and adaptive immunity. In our analysis of CD244 (2B4)-expressing cells in PBMCs from patients with active TB, a CD3(-)CD244(high) subpopulation was identified. A match of cell population in flow cytometry showed that nearly all CD3(-)CD244(high) cells were CD3(-)HLA-DR(-)CD11b(int)CD33(+) cells. The CD3-CD244(high) cell population has phenotypes of CD3(-)CD19(-)CD56(-)CD15(-)CD66b(-)CD33(+)CD11b(+)CD14(-)HLA-DRneg/low, which was consistent with MDSCs in humans as previously reported. Patients with active TB had higher frequencies of CD3(-)CD244(high) cells as compared with healthy controls. The CD3(-)CD244(high) cell population had high levels of NOS2 expression and was negatively correlated with activation and effective molecule production of CD4(+) and CD8(+) T cells. In conclusion, CD3(-)CD244(high) cells had phenotypes of MDSCs and CD244 might be used as a marker for human CD3(-)HLA(-)DR(-)CD11b(int)CD33(+) MDSCs. (C) 2013 Elsevier B.V. All rights reserved.